Effects of exogenous ATM gene on mRNA expression of human telomerase reverse transcriptase in AT cells induced by irradiation

SHENG Fangjun; CAO Jianping; LUO Jialin; ZHU Wei; LIU Fenju; FENG Shuang; SONG Jianyuan; LI Chong

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Effects of exogenous ATM gene on mRNA expression of human telomerase reverse transcriptase in AT cells induced by irradiation

更新时间：2023-03-30

- Effects of exogenous ATM gene on mRNA expression of human telomerase reverse transcriptase in AT cells induced by irradiation
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 5, Pages: 316-320(2005)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院放射生物学教研室　苏州　215123
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SHENG Fangjun, CAO Jianping, LUO Jialin, et al. Effects of exogenous ATM gene on mRNA expression of human telomerase reverse transcriptase in AT cells induced by irradiation. [J]. Journal of Radiation Research and Radiation Processing 23(5):316-320(2005)
DOI：

SHENG Fangjun, CAO Jianping, LUO Jialin, et al. Effects of exogenous ATM gene on mRNA expression of human telomerase reverse transcriptase in AT cells induced by irradiation. [J]. Journal of Radiation Research and Radiation Processing 23(5):316-320(2005) DOI：

摘要

通过观察外源性毛细血管扩张性共济失调症突变基因（Ataxia-telangiectasia mutation，ATM）对毛细血管扩张性共济失调症（Ataxia-telangiectasia，AT）患者皮肤的成纤维细胞系AT5BIVA（AT细胞）端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）mRNA表达的影响，探讨ATM对hTERT 的调控作用。采用RT-PCR法，对比ATM基因转染前、后AT细胞hTERT mRNA表达的变化及与源自正常人皮肤的成纤维细胞系GM0639（GM细胞）相比的差异；以及细胞经3Gy, 60,Co γ射线照射后其hTERT mRNA表达的变化。结果显示，未照射时，GM细胞hTERT mRNA表达呈阴性，AT细胞hTERT mRNA表达呈阳性，转染ATM基因后的ATM,+,-AT细胞其hTERT mRNA的表达明显下降（,p,<,0.05）；,60,Coγ射线照射后，GM细胞hTERT mRNA表达呈阳性，AT细胞、空载体AT细胞（PEBS7-AT细胞）和ATM,+,-AT细胞hTERT mRNA的表达量比未照射时明显增加（,p,<,0.05），ATM,+,-AT细胞hTERT mRNA相对表达量的增加低于AT细胞和空载体AT细胞（,p,<,0.05）。提示ATM可下调hTERT mRNA的表达；电离辐射可诱导细胞hTERT mRNA表达；端粒酶参与DNA损伤修复。

Abstract

The study is to observe effects of exogenous ATM gene on mRNA expression of hTERT (human telomerase reverse transcriptase) in fibroblast cells (AT5BIVA cells) from skin of Ataxia-telangiectasia (AT) patients and to study the regulation of ATM to hTERT. Using reverse transcription polymerase chain reaction (RT-PCR), mRNA expression of hTERT in AT、PEBS7-AT、ATM,+,-AT and GM cells irradiated with 0 and 3Gy of ,60,Co γ-rays were examined respectively. The difference of the mRNA expression of hTERT among AT、PEBS7-AT、ATM,+,-AT and GM cells were analyzed. Difference of the mRNA expression of hTERT between 0Gy and 3Gy groups was analyzed, too. The results showed that the mRNA expression of hTERT in GM cells was negative, but positive mRNA expression of hTERT in AT cells. The mRNA expression of hTERT in ATM,+,-AT cells decreased significantly (,p,<,0.05). After exposure to, 60,Co γ-rays, the mRNA expression of hTERT in GM cells was positive, and that in AT、PEBS7-AT、ATM,+,-AT cells was increased (,p,<,0.05). The increase of relative expression of hTERT mRNA in ATM,+,-AT cells was lower than that in AT and PEBS7-AT cells respectively (,p,<,0.05). It is postulated that exogenous ATM is able to downregulate the mRNA expression of hTERT in AT cells, ionizing radiation can induce the mRNA expression of hTERT in cells and telomerase anticipates the repair of damaged DNA.

关键词

毛细血管扩张性共济失调症毛细血管扩张性共济失调症突变基因端粒酶逆转录酶电离辐射

Keywords

Ataxia-telangiectasiaATM geneHuman telomerase reverse transcriptaseIonizing radiation

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