Application of <sup>60</sup>Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA

FENG Bo; ZENG Hongyan; TANG Yufang; WANG Lu

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Application of ⁶⁰Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA

更新时间：2023-03-30

- Application of ⁶⁰Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 6, Pages: 321-324(2005)
- 作者机构：
  
  1.湘潭大学食品与生物工程系　湘潭　411105
  2.武汉大学生命科学院　武汉　430072
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FENG Bo, ZENG Hongyan, TANG Yufang, et al. Application of ⁶⁰Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA. [J]. Journal of Radiation Research and Radiation Processing 23(6):321-324(2005)
DOI：

FENG Bo, ZENG Hongyan, TANG Yufang, et al. Application of ⁶⁰Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA. [J]. Journal of Radiation Research and Radiation Processing 23(6):321-324(2005) DOI：

摘要

为了探讨,60,Co γ射线辐照对聚苯乙烯微孔板吸附小分子多肽的影响，建立了检测抗−丙型肝炎病毒（Hepatitis C virus，HCV）的间接酶联免疫法（Enzyme−linked immunosorbent assay，ELISA）。分别用未经辐照处理、紫外线（Ultraviolet ray，UV）辐照处理和,60,Co辐照处理的微孔板吸附重组HCV抗原，再配以辣根过氧化物酶标记的羊抗人IgG，考察其灵敏度、特异性、均一性和稳定性等相关指标。实验结果表明，经8kGy,60,Co辐照处理的聚苯乙烯微孔板可改善酶联免疫法的测定效果，检测灵敏度和均一性均有所提高。

Abstract

In order to explore the effect of ,60,Coγ−ray irradiation on minor polypeptides absorption of polystyrene microplate, an indirect ELISA detection of anti−HCV was established,60,Co γ−ray irradiated polystyrene microplates and the controls (without irradiation or UV−irradiated) were applied to absorb recombinant HCV antigens respectively. Cooperated with Bovine antihuman IgG labed HRP, their related indices of sensitivity, specificity, homogeneity and stability were determinated. The results indicated that, optimum dose of the γ−ray irradiation is 8kGy, and compared with the controls, detection sensitivity and homogeneity of the polystyrene microplate irradiated to 8kGy could be improved markedly.

关键词

酶联免疫法60Co γ射线小分子多肽重组丙型肝炎病毒抗原

Keywords

ELISA60Coγ−rayMinor polypeptidesRecombinant HCV antigens

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Application of 60Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA

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Application of ⁶⁰Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA