Effect of tamoxifen combined with <sup>60</sup>Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells

NING Ping; LIU Fenju; XUE Jing

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Effect of tamoxifen combined with ⁶⁰Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells

更新时间：2023-03-30

- Effect of tamoxifen combined with ⁶⁰Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 3, Pages: 183-187(2006)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　江苏苏州　215123
  2.江苏省放射医学与防护重点实验室　江苏苏州　215123
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NING Ping, LIU Fenju, XUE Jing. Effect of tamoxifen combined with ⁶⁰Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells. [J]. Journal of Radiation Research and Radiation Processing 24(3):183-187(2006)
DOI：

NING Ping, LIU Fenju, XUE Jing. Effect of tamoxifen combined with ⁶⁰Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells. [J]. Journal of Radiation Research and Radiation Processing 24(3):183-187(2006) DOI：

摘要

研究三苯氧胺（Tamoxifen，TAM）对人脑胶质瘤细胞辐射增敏作用及诱导细胞凋亡。用,3,H-TdR掺入法、免疫组织化学法、流式细胞术和激光共聚焦显微镜扫描技术检测细胞DNA合成、雌激素受体表达、细胞凋亡及凋亡的形态学特征。结果表明，TAM能抑制细胞DNA合成，与,60,Co γ线联用抑制作用增强，表现出协同作用。TAM能诱导细胞凋亡，其凋亡率随TAM浓度的升高而增高，凋亡细胞核固缩和核碎裂，可见凋亡小体。而免疫组化结果显示SHG-44细胞不表达雌激素受体。以上结果表明TAM可能通过诱导细胞凋亡途径来增加细胞的辐射敏感性，而与雌激素受体途径无关。

Abstract

To study radiosensitizing effect and apoptosis induction of tamoxifen on human glioma cells, 3,H-TdR incorporation assay, flow cytometry, laser confocal microscope and Immunohistochemistry were used to investigate the inhibition effect on DNA synthesis, apoptosis and estrogen receptor expression. Experiment results suggested that tamoxifen could inhibit DNA synthesis in SHG-44 cells,the combination of tamoxifen and ,60,Co γ-irradiation showed a synergistic action on it. Tamoxifen could also induce the apoptosis, and the apoptosis rate increased along with tamoxifen concentration was elevated, so morphologyical changes, such as karyopyknosis, nuclear fragmentation and apoptotic body were observed. However, SHG-44 cells did not express estrogen receptor. The above results revealed that tamoxifen can sensitize SHG-44 cells to gama radiation, which might be related with apoptosis pathway instead of the estrogen receptor-mediated process.

关键词

三苯氧胺脑胶质瘤细胞辐射增敏凋亡

Keywords

TamoxifenGliomaRadiosensitivityApoptosis

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Effect of tamoxifen combined with 60Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells

DOI：

摘要

Abstract

关键词

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references

Effect of tamoxifen combined with ⁶⁰Co γ-irradiation on proliferation inhibition and apoptosis induction in human glioma SHG-44 cells