Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N<sup>+</sup> ion beam implantation

FANG Chuanji; LU Zhaoxin; SUN Lijun; LV Fengxia; BIE Xiaomei

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Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N⁺ ion beam implantation

更新时间：2023-03-30

- Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N⁺ ion beam implantation
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 6, Pages: 333-336(2006)
- 作者机构：
  
  1.南京农业大学食品科技学院　南京　210095
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FANG Chuanji, LU Zhaoxin, SUN Lijun, et al. Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N⁺ ion beam implantation. [J]. Journal of Radiation Research and Radiation Processing 24(6):333-336(2006)
DOI：

FANG Chuanji, LU Zhaoxin, SUN Lijun, et al. Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N⁺ ion beam implantation. [J]. Journal of Radiation Research and Radiation Processing 24(6):333-336(2006) DOI：

摘要

采用了低能N,+,离子注入技术，以淀粉液化芽孢杆菌（,Bacillus amyloliquefaciens,）ES-2为出发菌株，进行诱变选育抗菌脂肽高产菌株。研究了不同注入剂量对存活率和正突变率的影响，并获得一株抗菌脂肽高产菌，命名为淀粉液化芽孢杆菌（,B.amyloliquefaciens,）ES-2-4。与原始菌株相比，脂肽含量提高了15.2%。此外，对突变株的发酵特性进行了初步研究。结果表明：突变株（,B.amyloliquefaciens,）ES-2-4的延滞期短，稳定期长，脂肽产量高，其发酵性能有利于脂肽的大规模生产。

Abstract

Bacillus amyloliquefaciens, ES-2 was implanted with 20keV N,+, ion beam to breed mutants of high yield of antimicrobial lipopeptides. The dose effects of the nitrogen ion implantation on the survival and positive mutations rate was studied. The mutant strain designated as ,B. amyloliquefaciens, ES-2-4 showing higher yield of antimicrobial lipopeptides was obtained. The concentration of the lipopeptides in fermentation liquid increased by 15.2% compared with the original strain. We also studied the fermentation process between the mutant and the original strain. It indicated that the mutant with shorter lag phase, longer stable phase and higher yield of the lipopeptides, which was suitable for large-scale production.

关键词

离子注入脂肽诱变选育发酵

Keywords

Ion implantationAntimicrobial lipopeptidesMutation breedingFermentation

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Ion Beam Bio-engineering Center, Xinjiang University

College of Life Science and Pharmacy, Nanjing University of Technology

Longping Branch, Graduate School of Hunan University

Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences/ Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation

Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, Institute of Advanced Materials Physics

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Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N+ ion beam implantation

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摘要

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Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N⁺ ion beam implantation