Enhancement of wortmannin on <sup>60</sup>Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells

XUE Jing; LIU Fenju; YU Jiahua; LI Bingyan; YANG Xueqin; SUN Zhiqiang

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Enhancement of wortmannin on ⁶⁰Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells

更新时间：2023-03-30

- Enhancement of wortmannin on ⁶⁰Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 6, Pages: 373-377(2006)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215123
  2.苏州市立医院　苏州　215004
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XUE Jing, LIU Fenju, YU Jiahua, et al. Enhancement of wortmannin on ⁶⁰Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells. [J]. Journal of Radiation Research and Radiation Processing 24(6):373-377(2006)
DOI：

XUE Jing, LIU Fenju, YU Jiahua, et al. Enhancement of wortmannin on ⁶⁰Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells. [J]. Journal of Radiation Research and Radiation Processing 24(6):373-377(2006) DOI：

摘要

研究渥曼青霉素（Wortmannin, WORT）对人脑胶质瘤细胞辐射增敏作用及诱导细胞凋亡。用克隆法、流式细胞术和激光共聚焦显微镜扫描技术检测细胞克隆形成、细胞周期和凋亡以及凋亡的形态学特征。结果表明：WORT能抑制细胞克隆形成，和,60,Co γ射线联用抑制作用增强，表现出协同作用。WORT和,60,Co γ射线联用能引起SHG-44细胞G,1,期阻滞，WORT能增强,60,Co γ射线诱导的细胞凋亡，凋亡细胞核固缩和核碎裂，可见凋亡小体。以上结果表明，WORT可能通过诱导细胞G,1,期阻滞和凋亡途径来增加细胞的辐射敏感性。

Abstract

To study radiosensitizing effect and apoptosis induction of wortmannin on human glioma cells, colony formation assay, flow cytometry, laser confocal microscope were used to investigate the inhibition effect on colony formation, apoptosis and cell cycle procession. Experiment results suggested that wortmannin could inhibit colony formation in SHG-44 cells, and the combination of wortmannin with ,60,Co γ-irradiation showed a synergistic action. Wortmannin could also induce G,1, arrest, apoptosis, and morphologyical changes such as karyopyknosis, nuclear-fragmentation and apoptotic body were observed. The above results revealed that wortmannin can sensitize SHG-44 cells to gama radiation, which might be related with G,1, arrest and apoptosis pathway.

关键词

渥曼青霉素脑胶质瘤细胞辐射增敏凋亡

Keywords

WortmanninGliomaRadiosensitivityApoptosis

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The Key Laboratory of Radiation Medicine and Protection of Jiangsu Province, Suzhou University

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Enhancement of wortmannin on 60Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells

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Abstract

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Enhancement of wortmannin on ⁶⁰Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells