Preliminary study on flower color mutant induced by 80MeV/u <sup>12</sup>C<sup>+6</sup> ions in <italic style="font-style: italic">Dahlia pinnata Cav</italic> using RAPD technique

DONG Xicun; LI Wenjian; YU Lixia; ZHOU Libin; MA Shuang; XIE Hongmei

您当前的位置：

首页 >

文章列表页 >

Preliminary study on flower color mutant induced by 80MeV/u ¹²C⁺⁶ ions in Dahlia pinnata Cav using RAPD technique

更新时间：2023-03-30

- Preliminary study on flower color mutant induced by 80MeV/u ¹²C⁺⁶ ions in Dahlia pinnata Cav using RAPD technique
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 1, Pages: 62-64(2007)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.中国科学院研究生院　北京　100049
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
DONG Xicun, LI Wenjian, YU Lixia, et al. Preliminary study on flower color mutant induced by 80MeV/u ¹²C⁺⁶ ions in Dahlia pinnata Cav using RAPD technique. [J]. Journal of Radiation Research and Radiation Processing 25(1):62-64(2007)
DOI：

DONG Xicun, LI Wenjian, YU Lixia, et al. Preliminary study on flower color mutant induced by 80MeV/u ¹²C⁺⁶ ions in Dahlia pinnata Cav using RAPD technique. [J]. Journal of Radiation Research and Radiation Processing 25(1):62-64(2007) DOI：

摘要

品系为“大雪青”的大丽花幼枝，经兰州重离子加速器提供的80MeV/u ,12,C,6+,束辐照后，种植在甘肃省定西市临洮新兴花卉公司基地。辐照8Gy的幼枝有一株花色突变体，用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对突变体和野生型进行检测分析。琼脂糖凝胶电泳结果表明，所用的10条引物中，有7条引物共扩增出78条带，其中5条引物扩增出了11条多态性片段，从而在DNA水平上证实了两者之间存在着差异，为进一步探讨重离子诱变机理打下基础。

Abstract

Young shoots of ,Dahlia pinnata Cav, (Daxueqing) cultivar were irradiated by 80MeV/u ,12,C,6+, ions. The as-irradiated shoots were planted in the field of Lintao Flower Corporation in Dingxi City, Gansu Province. Flower color mutant was induced by 8Gy irradiation. Mutant and wild type of ,Dahlia pinnata Cav, were detected for their DNA level differences using RAPD (Random amplified polymorphic DNA) method. The spectra of electrophoresis in agarose gel indicated that among 10 primers used, the 78 bands are produced in 7 primers. Among them 11 polymorphic bands are produced in 5 primers, which substantiated the differences in the DNA level between them. Mutation mechanism by heavy ion beams will be further studied on basis of the results.

关键词

碳离子束花色突变体大丽花随机扩增多态性DNA

Keywords

Carbon ion beamFlower color mutantDahlia pinnata CavRandom amplified polymorphic DNA

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors

Detection of DNA damage of Lewis lung carcinoma cells irradiated by carbon ion beam and X-rays with comet assay

Related Author

No data

Related Institution

Department of Radiation Oncology, Gansu Provincial Cancer Hospital

Gansu Provincial Academy of Medical Sciences

⁰

Preliminary study on flower color mutant induced by 80MeV/u 12C+6 ions in Dahlia pinnata Cav using RAPD technique

DOI：

摘要

Abstract

关键词

Keywords

references

Preliminary study on flower color mutant induced by 80MeV/u ¹²C⁺⁶ ions in Dahlia pinnata Cav using RAPD technique