Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions

YANG Yingge; LI Wen; LIU Dan; FAN Yonghong; WANG Dongmei; ZHENG Zhiming; YU Zengliang

您当前的位置：

首页 >

文章列表页 >

Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions

更新时间：2023-03-30

- Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 2, Pages: 80-84(2007)
- 作者机构：
  
  1.中国科学院等离子体物理研究所离子束生物工程重点实验室　合肥　230031
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
YANG Yingge, LI Wen, LIU Dan, et al. Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions. [J]. Journal of Radiation Research and Radiation Processing 25(2):80-84(2007)
DOI：

YANG Yingge, LI Wen, LIU Dan, et al. Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions. [J]. Journal of Radiation Research and Radiation Processing 25(2):80-84(2007) DOI：

摘要

为了获得能够高效发酵木糖生产L(+)－乳酸的菌株，采用氮离子注入诱变方法，对出发菌株米根霉RLC41－6进行改良，获得高产L(+)－乳酸菌株RQ4012，发酵周期为72h，产酸能力达74.37g/L，产酸速率达1.03g/(L.h)，比RLC41－6提高了1.6倍。经过多次传代实验证明该菌株具有较好的遗传稳定性。

Abstract

In order to obtain higher L(+)-lactic acid yield strain fermentating from xylose, the original strain ,Rhizopus oryzae, RLC41-6 was mutated by 10keV N,+, ion implantation. A mutant strain RQ4012 was obtained. After 72h shake-flask cultivation, the concentration of L(+)-lactic acid reached 74.37g/L, and the productivity was 1.03g/(L.h). Its lactic acid yield was 160% higher than that of the original one, and the mutant strain has high genetic stability.

关键词

离子注入米根霉木糖L(+)－乳酸

Keywords

Ions implantationRhizopus oryzaeXyloseL(+)-lactic acid

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The construction of survival-dose effect model of B.trispora (−) implanted by low energy N⁺

Related Author

No data

Related Institution

Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key and Open Lab on Forest Chemical Engineering, State Forestry Administration

Nanjing University of Technology

⁰