UV-Radiation induced oxidative damage modification by cholesterol in phosphatidylcholine and liposomal membrane

WANG Ning; WANG Weidong; ZHU Xiaorui; WANG Junling; ZHANG Dawei; QIN Guangyong

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UV-Radiation induced oxidative damage modification by cholesterol in phosphatidylcholine and liposomal membrane

更新时间：2023-03-30

- UV-Radiation induced oxidative damage modification by cholesterol in phosphatidylcholine and liposomal membrane
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 2, Pages: 115-118(2007)
- 作者机构：
  
  1.河南省离子束生物工程重点实验室，河南郑州大学物理工程学院　郑州　450052
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WANG Ning, WANG Weidong, ZHU Xiaorui, et al. UV-Radiation induced oxidative damage modification by cholesterol in phosphatidylcholine and liposomal membrane. [J]. Journal of Radiation Research and Radiation Processing 25(2):115-118(2007)
DOI：

WANG Ning, WANG Weidong, ZHU Xiaorui, et al. UV-Radiation induced oxidative damage modification by cholesterol in phosphatidylcholine and liposomal membrane. [J]. Journal of Radiation Research and Radiation Processing 25(2):115-118(2007) DOI：

摘要

以卵磷脂(L-α-phosphatidylcholine)和以卵磷脂制备的单层脂质体为材料，研究紫外线引发磷脂和脂质体膜的过氧化反应以及胆固醇对过氧化反应的保护作用。用20μmol的L-α-phosphatidylcholine和胆固醇按一定的摩尔比混合制备的磷脂溶液和脂质体，经过紫外线照射后，用硫代巴比妥酸(Thiobarbituric acid，TBA)法检测接受照射的样品中过氧化产物的量。结果表明，磷脂和脂质体过氧化程度随紫外线照射量增加，且由卵磷脂制备的单层脂质体对辐射的敏感性明显强于磷脂。含胆固醇的脂质体的过氧化程度明显低于不含胆固醇，而胆固醇对磷脂无明显保护作用。磷脂和脂质体的过氧化程度与其浓度和照射量都相关，而胆固醇只对脂质体有保护作用。

Abstract

In order to study the peroxidation reaction of phosphatidylcholine and liposome induced by UV and the protection of cholesterol to phosphatidylcholine and liposome, predominantly unilamellar vesicles (ULVs) prepared by L-α-phosphatidylcholine were used as an alternate biological model. Phosphatidylcholine and liposome made by 20μmol of L-α-phosphatidylcholine with or without cholesterol were irradiated by 254 nm UV ray. Radiation induced peroxidation in phosphatidylcholine and liposome was measured spectrophotometrically by thiobarbituric acid (TBA) reaction. The results show that, the peroxidation of phosphatidylcholine and liposome increased with the UV irradiation dose. The liposome was more sensitive to UV radiation than phosphatidylcholine. Cholestrol significantly prevented Liposomal peroxidation, but had no effect on phosphatidylcholine peroxidation. The peroxidation of phosphatidylcholine and liposome is apparently related to the concentration and dose, and cholesterol can only protect liposome.

关键词

紫外辐射脂质体脂质过氧化卵磷脂

Keywords

UV-RadiationLiposomeLipid-peroxidationPhosphatidylcholine

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