Effect of TGF-β1 on early remodeling of pulmonary injury following irradiation

DIAO Ruiying; SONG Liangwen; WANG Shaoxia; LI Ming; XU Xinping

您当前的位置：

首页 >

文章列表页 >

Effect of TGF-β1 on early remodeling of pulmonary injury following irradiation

更新时间：2023-03-30

- Effect of TGF-β1 on early remodeling of pulmonary injury following irradiation
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 3, Pages: 189-192(2007)
- 作者机构：
  
  1.军事医学科学院放射与辐射医学研究所　北京　100850
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
DIAO Ruiying, SONG Liangwen, WANG Shaoxia, et al. Effect of TGF-β1 on early remodeling of pulmonary injury following irradiation. [J]. Journal of Radiation Research and Radiation Processing 25(3):189-192(2007)
DOI：

DIAO Ruiying, SONG Liangwen, WANG Shaoxia, et al. Effect of TGF-β1 on early remodeling of pulmonary injury following irradiation. [J]. Journal of Radiation Research and Radiation Processing 25(3):189-192(2007) DOI：

摘要

探讨TGF-β1在放射性肺损伤早期肺组织重建中的作用。用酶标记免疫吸附测定法（Enzyme-labeled immunosorbent assay，ELISA）检测照射后不同时期（1、2和4周）大鼠血清中TGF-β1的水平变化；用甲基噻唑基四唑比色法（Methyl thiazolyl tetrazolium colourimetry，MTT）检测照射后4周大鼠血清对人肺成纤维细胞（Human lung fibroblast，HLF）的促增殖作用；用ELISA检测HLF经大鼠血清刺激后合成分泌MMP-9（Matrix metalloproteinases-9, MMP-9）和Ⅳ型胶原的作用。照射后不同时间提取的大鼠血清中TGF-β1水平从2至4周逐渐增加；照射后大鼠血清能促进HLF增殖，10%血清浓度的培养液促细胞增殖的作用最强；受照后大鼠血清不但能促进Fb增生，而且能显著促进HLF合成释放IV型胶原和MMP-9。放射性肺损伤早期肺组织TGF-β1表达增加，进而促进肺成纤维细胞增生、合成和释放IV型胶原和MMP-9，参与早期肺损伤的组织重建过程。

Abstract

To investigate the role of TGF-β1 on the early lung remodeling after radiation pulmonary injury. The dose-effect of human lung fibroblast proliferation promoted by serum after radiated rat was observed by MTT colorimetric methods. ELISA was used to observe the contents of TGF-β1 in serum as well as the expression of collagen IV and MMP-9 in HLF stimulated by serum after radiated rat. The level of TGF-β1 in rat serum increased from 2 to 4 weeks after irradiation. HLF proliferation was significantly promoted by the serum of irradiated rat at a dose of 10% in the medium. The serum of irradiated rat could not only promote HLF proliferation, but also increase the expressions of collagen IV and MMP-9 in HLF. TGF-β1 plays an important role in the proliferation of HLF and the expressions of collagen IV and MMP-9 in HLF, and is involved in the process of lung remodeling after radiation pulmonary injury.

关键词

TGF-β1MMP-9Ⅳ型胶原肺重建成纤维细胞

Keywords

TGF-β1MMP-9Collagen type IVPulmonary remodelingFibroblast

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Bioinformatic analysis of differentially expressed genes in tumor-associated fibroblasts after ionizing radiation

Effects and mechanism of CpG-ODN ameliorating radiation induced pulmonary fibrosis

TGF-β1 inhibitors increasing the radiosensitivity of H460 lung cancer cells

Protective effects of mengshan green tea and hawk tea against UV-ray irradiation

THE CHARACTERS OF PATHOLOGY AND ULTRASTRUCTURE OF FIBROBLASTS IN NORMAL WOUND AND RADIATION-COMBINED WOUND HEALING

Related Author

No data

Related Institution

Department of Basic Medicine, Yongzhou Vocational Technical College

Department of Pathology, the First Affiliated Hospital of Hunan University of Medicine

Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine

Department of Radiotherapy, Lanzhou General Hospital of PLA

Faculty of Naval Medicine, The Second Military Medical University

⁰