Effect of combination of AdCMV-p53 and irradiation on tumor cells

LIU Bing; ZHANG Hong; GAO Qingxiang; LUO Xiaohong; XIE Yi; HAO Jifang; DUAN Xin; ZHOU Qingming; ZHOU Guangming

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Effect of combination of AdCMV-p53 and irradiation on tumor cells

更新时间：2023-03-30

- Effect of combination of AdCMV-p53 and irradiation on tumor cells
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 3, Pages: 175-179(2007)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.中国科学院研究生院　北京　100039
  3.兰州军区疾病控制中心　兰州　730020
  4.兰州大学生命科学院　兰州　730000
- 作者简介：
- 基金信息：
- DOI：
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LIU Bing, ZHANG Hong, GAO Qingxiang, et al. Effect of combination of AdCMV-p53 and irradiation on tumor cells. [J]. Journal of Radiation Research and Radiation Processing 25(3):175-179(2007)
DOI：

LIU Bing, ZHANG Hong, GAO Qingxiang, et al. Effect of combination of AdCMV-p53 and irradiation on tumor cells. [J]. Journal of Radiation Research and Radiation Processing 25(3):175-179(2007) DOI：

摘要

以绿色荧光蛋白腺病毒重组体（Replication deficient adenovirus green fluorescence protein recombinant，AdCMV-GFP）为对照，用p53腺病毒重组体（Replication deficient adenovirus p53 recombinant，AdCMV-p53）转染经0、0.25、0.5、1.0、1.5和2.0Gy γ射线预辐射的HepG2 (,wt,p53)、Hela (,wt,p53，,wt,P53低水平表达)和HT-29（,mt,p53，,mt,P53过表达）细胞，用克隆形成法检测肿瘤细胞存活，探讨AdCMV-p53转染对p53基因状态与功能不同肿瘤细胞辐射敏感性的影响。结果显示，AdCMV-p53转染不仅明显提高肿瘤细胞辐射敏感性，而且与肿瘤细胞内在p53基因状态与功能有关。

Abstract

The work is to investigate effect of AdCMV-p53 gene transfer on the radiosensitivity of tumor cells. HepG2 (,wt,p53), Hela (,wt,p53, low level ,wt,p53 expression) and HT-29 (,mt,p53,mt,p53 over-expression) cells exposed to 0, 0.25, 0.5, 1.0, 1.5 and 2.0Gy were infected with AdCMV-GFP, a replication deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein, or AdCMV-p53, a replication deficient recombinant adenoviral vector containing a CMV promoter and carrying human wild-type p53 gene. Survival fraction of the cells was determined by clonogenic assay. Survival curves were generated by linear quadratic model. The results showed that AdCMV-p53 infection could significantly promote the radiosensitivity of tumor cells. There is a relationship between the effect of AdCMV-p53 infection and the cellular intrinsic p53 status.

关键词

p53基因辐射腺病毒载体肿瘤细胞辐射敏感性

Keywords

p53IrradiationAdenovirus vectorTumor cellRadiosensitivity

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