Protective and repairing abilities of fusion protein PTD-SOD for L-02 cell irradiated by UV-C

ZHANG Chen; CHEN Xiaochao; LIU Shutao; LIU Yuangang; RAO Pingfan

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Protective and repairing abilities of fusion protein PTD-SOD for L-02 cell irradiated by UV-C

更新时间：2023-03-30

- Protective and repairing abilities of fusion protein PTD-SOD for L-02 cell irradiated by UV-C
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 4, Pages: 248-251(2007)
- 作者机构：
  
  1.福州大学生物工程研究所　福州　350002
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ZHANG Chen, CHEN Xiaochao, LIU Shutao, et al. Protective and repairing abilities of fusion protein PTD-SOD for L-02 cell irradiated by UV-C. [J]. Journal of Radiation Research and Radiation Processing 25(4):248-251(2007)
DOI：

ZHANG Chen, CHEN Xiaochao, LIU Shutao, et al. Protective and repairing abilities of fusion protein PTD-SOD for L-02 cell irradiated by UV-C. [J]. Journal of Radiation Research and Radiation Processing 25(4):248-251(2007) DOI：

摘要

通过体外细胞培养，探讨了融合蛋白PTD-SOD是否径由跨膜转导而增强SOD对靶细胞紫外辐射损伤防护和修复能力。结果表明辐照时间为120s，酶活力为320U/mL时，PTD-SOD对细胞的保护率达58.5%，约为野生型SOD的5倍；紫外辐照时间为140s，酶活力为500U/mL时，PTD-SOD对细胞的保护率仍有43.2%，相同情况下野生型SOD的保护率趋近于0；辐照时间40s，酶活力为500U/mL时PTD-SOD对细胞的修复率有50.1%，而野生型SOD的修复率最高也没有超过8%。这些结果说明相对野生型SOD，PTD-SOD对L-02细胞的保护和修复能力得到了显著的增强，能有效地提高SOD的生物利用率。

Abstract

Cell culture was carried out to investigate whether the protective and repairing capacity of SOD against the radiation damage induced by UV-C was improved because of its transduction ability. When L-02 cell line being exposed to UV-C in the solution with SOD enzymatic activity of 320U/mL, the protective rate of PTD-SOD increased to 58.5% , which was about 5 times of that of wild-type SOD; when irradiation duration prolonged to 140s, the protective rate of PTD-SOD remained to be 43.2%, while that of wild-type SOD was almost undetectable. Regarding restorative rate, that of PTD-SOD could reserve 50.1% when L-02 cell exposed to UV-C for 40s, while that of wild-type SOD was less than 8%. These results suggested PTD enhanced the protective and repairing ability of SOD against UV radiation remarkably, owing to improve bioavailability of SOD.

关键词

蛋白质转导域氧化歧化酶紫外辐射

Keywords

Protein transduction domainSuperoxide dismutaseUltraviolet

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