THE STUDY OF RELATIONSHIP BETWEEN RADIATION—INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY

ZHU Hanneng; SUN Daren; LUO Weihua; SHI Jianhui; CHENG Wenying

您当前的位置：

首页 >

文章列表页 >

THE STUDY OF RELATIONSHIP BETWEEN RADIATION—INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY

更新时间：2023-04-07

- THE STUDY OF RELATIONSHIP BETWEEN RADIATION—INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY
- Journal of Radiation Research and Radiation Processing Vol. 17, Issue 2, Pages: 107-110(1999)
- 作者机构：
  
  1.上海医科大学放射医学研究所　上海　200032
- 作者简介：
- 基金信息：
  
  Scientific Foundation of National Health Bureau(96—1—159)
- DOI：
  CLC：
扫描看全文
ZHU Hanneng, SUN Daren, LUO Weihua, et al. THE STUDY OF RELATIONSHIP BETWEEN RADIATION—INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY. [J]. Journal of Radiation Research and Radiation Processing 17(2):107-110(1999)
DOI：

ZHU Hanneng, SUN Daren, LUO Weihua, et al. THE STUDY OF RELATIONSHIP BETWEEN RADIATION—INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY. [J]. Journal of Radiation Research and Radiation Processing 17(2):107-110(1999) DOI：

摘要

为探讨不同放射敏感性人成纤维细胞株经辐射诱导后的凋亡情况，应用Tunel法及流式细胞术（FCM）检测细胞凋亡，以,60,Co γ射线处理细胞。结果表明（1）,60,Co γ射线照射后，集落形成实验表明，6号细胞株辐射敏感性高于8号细胞株。（2）Tunel法和流式细胞术检测结果表明，凋亡率与辐射敏感性成正比，同样反映出6号细胞株辐射敏感性高于8号细胞株。（3）检测还发现，凋亡率与辐射剂量和辐射后时间成正比。说明凋亡率的变化能够反映细胞株的辐射敏感性水平；凋亡率与辐射剂量和时间呈依赖关系，有可能成为推断辐射敏感性的重要指标之一。

Abstract

To investigate the radiation — induced apoptosis of human fibroblasts with different radiosensitivity, the methods of TUNEL and FCM were employed to test the apoptosis of cells treated with ,60,Co ,γ,—rays. The results of experiments indicated that : (1) Surviving fraction experiment identified the No 6 fibroblast cells have a high radiosensitivity than that of No 8 cells after exposured to ,60,Co ,γ,—rays; (2) Apoptosis rate has a positive relationship with radiosensitivity by the methods of TUNEL and FCM, in accordance with the result made by the surviving fraction experiment; (3) Both apoptosis rate and radiosensitivity increased within the 48 hours after radiation. In this conclusion is that : The changing of apoptosis rate can reflect the radiosensitivity level of cell line; the changing of cellular apoptosis rate and radiosensitivity were time—dependant after radiation. it is possible that apoptosis may be an important marker for judging cellular radiosensitivity.

关键词

人成纤维细胞株凋亡辐射敏感性

Keywords

Human fibroblastApoptosisRadiosensitivity

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of CpG-ODN on spleen damage after carbon ion radiation

Protective effects of pigment epithelium-derived factor against X-ray-induced retinopathy

Effects of millimeter wave radiation on thereproductive system of male mice

Ionizing radiation promotes primary ciliogenesis in glioblastoma cells

Effect of CpG-ODN on thymus damage radiated by carbon ions

Related Author

No data

Related Institution

Faculty of Naval Medicine, Naval Military Medical University

Department of Radiotherapy, The Hospital of Joint Logistic Support Force of PLA

School of Electronic and Information Engineering, Soochow University

State Key Laboratory of Radiation Medicine and Protection

School of Radiation Medicine and Protection, Medical College of Soochow University

⁰