THE EFFECTS OF LOW DOSE IONIZING RADIATION ON THE ACTIVITY OF CuZn SOD IN PERITONEAL MACROPHAGES OF MICE

JIN Shunzi; HE Shumei; LIU Shuzheng

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THE EFFECTS OF LOW DOSE IONIZING RADIATION ON THE ACTIVITY OF CuZn SOD IN PERITONEAL MACROPHAGES OF MICE

更新时间：2023-04-07

- THE EFFECTS OF LOW DOSE IONIZING RADIATION ON THE ACTIVITY OF CuZn SOD IN PERITONEAL MACROPHAGES OF MICE
- Journal of Radiation Research and Radiation Processing Vol. 20, Issue 3, Pages: 209-212(2002)
- 作者机构：
  
  1.吉林大学卫生部放射生物学重点实验室　长春　130021
  2.吉林大学第一医院干部病房　长春　130021
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(39570188)
- DOI：
  CLC：
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JIN Shunzi, HE Shumei, LIU Shuzheng. THE EFFECTS OF LOW DOSE IONIZING RADIATION ON THE ACTIVITY OF CuZn SOD IN PERITONEAL MACROPHAGES OF MICE. [J]. Journal of Radiation Research and Radiation Processing 20(3):209-212(2002)
DOI：

JIN Shunzi, HE Shumei, LIU Shuzheng. THE EFFECTS OF LOW DOSE IONIZING RADIATION ON THE ACTIVITY OF CuZn SOD IN PERITONEAL MACROPHAGES OF MICE. [J]. Journal of Radiation Research and Radiation Processing 20(3):209-212(2002) DOI：

摘要

采用MTT法观察了低剂量电离辐射对小鼠腹腔巨噬细胞铜锌超氧化物歧化酶（CuZn SOD）活性的影响，同时用较大剂量作为对照，观察两者的不同之处，而且还利用印迹杂交方法观察了CuZn SOD的mRNA水平变化。结果表明，从转录水平上低剂量电离辐射可导致小鼠腹腔巨噬细胞CuZn SOD mRNA水平下降，而CuZn SOD活性却无明显改变；较高剂量电离辐射使小鼠腹腔巨噬细胞CuZn SOD活性在照射后8—12h明显增强。

Abstract

To observe the effects of low dose radiation (LDR) on the activity of the CuZn superoxide dismutase (CuZn SOD) in the peritoneal macrophages of mice, we used blot hybridization and MTT methods to detect the changes of this enzyme in the peritoneal macrophage of mice irradiated with 0.075Gy of X rays at both the mRNA level and the protein level (activity). The results showed that after irradiation with LDR the CuZn SOD mRNA expression in the macrophages increased, but the CuZn SOD activity had no noticeable change. However from 8 to 24h after irradiation with 2Gy,the CuZn SOD activity in the macrophages increased obviously.

关键词

电离辐射CuZn SOD小鼠腹腔巨噬细胞mRNA

Keywords

Ionizing radiationMouse Peritoneal macrophageCuZn SODmRNA

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