THE REGULATION EFFECT OF STAT5 SIGNALING PATHWAY ON THE CELL CYCLE PROGRESSION OF IRRADIATED KG-1 CELLS

GUO Dehuang; DONG Bo; LUO Qinglinang; WEN Gengyun; MAO Bingzhi

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THE REGULATION EFFECT OF STAT5 SIGNALING PATHWAY ON THE CELL CYCLE PROGRESSION OF IRRADIATED KG-1 CELLS

更新时间：2023-04-13

- THE REGULATION EFFECT OF STAT5 SIGNALING PATHWAY ON THE CELL CYCLE PROGRESSION OF IRRADIATED KG-1 CELLS
- Journal of Radiation Research and Radiation Processing Vol. 18, Issue 4, Pages: 299-303(2000)
- 作者机构：
  
  1.军事医学科学院放射医学研究所一室　北京　100850
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GUO Dehuang, DONG Bo, LUO Qinglinang, et al. THE REGULATION EFFECT OF STAT5 SIGNALING PATHWAY ON THE CELL CYCLE PROGRESSION OF IRRADIATED KG-1 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(4):299-303(2000)
DOI：

GUO Dehuang, DONG Bo, LUO Qinglinang, et al. THE REGULATION EFFECT OF STAT5 SIGNALING PATHWAY ON THE CELL CYCLE PROGRESSION OF IRRADIATED KG-1 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(4):299-303(2000) DOI：

摘要

探讨信号转导和转录激活子5（STAT5）信号转导途径在受辐射KG-1细胞中对细胞周期的调控作用。通过转染STAT5基因的显性负突变体（DN-STAT5）阻断JAK/STAT的信号传递后，瞬间转染cyclin D,1,基因及cyclin B,1,基因，观察cyclin D,1,蛋白及cyclin B,1,蛋白对受辐射细胞周期阻滞的影响作用。转染DN-STSAT5基因的受辐射KG-1细胞即使用粒–巨噬细胞集落刺激因子（GM-CSF）刺激亦不能跳出G,1,期阻滞；瞬间转染cyclin D,1,基因及cyclin B,1,基因分别能促进受辐射细胞跳出G,1,期和G,2,期阻滞。GM-CSF所激活的JAK/STAT信号转导途径通过促进周期蛋白cyclin D,1,及cyclin B,1,的表达而对受辐射细胞周期进行调控。

Abstract

This study investigated the role of the JAK/STAT signaling pathway regulating cell cycle progression in the irradiated KG-1 cells. By permanent transfecting the cells with DN-STAT5 cDNA to block the JAK/STAT signaling pathway and then transient transfecting with cyclin D,1, or cyclin B,1, cDNA, the effects of cyclin D,1, protein and cyclin B,1, protein on the cell cycle progression were examined. Results showed that after irradiation with 8Gy ,60,Co γ rays, the irradiated KG-1 cells transfected with only DN-STAT5 cDNA can not recover form the G,1, arrest, even though GM-CSF was added. Meanwhile, the cells transfected with both the DN-STAT5 cDNA and cyclin D,1, cDNA or cyclin B,1, cDNA can recover from the G,1, arrest or the G,2, arrest to a great extent. Thus, it was proved indirectly that the JAK/STAT signaling pathway activated by GM-CSF regulated the cell cycle progression through cyclin D,1, and cyclin B,1, protein.

关键词

辐射细胞周期周期蛋白D1周期蛋白B1

Keywords

IrradiationCell cycleCyclin D1Cyclin B1

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