AN EXPERIMENTAL STUDY ON THE ENHANCED MACROPHAGE (Mϕ) FUNCTION IN TUMOR-BEARING MICE TREATED WITH LOW DOSE RADIATION

Zhang Ying; Sun Yimin; Li Xiuyi; Mu Ying; Liu Shuzheng

您当前的位置：

首页 >

文章列表页 >

AN EXPERIMENTAL STUDY ON THE ENHANCED MACROPHAGE (Mϕ) FUNCTION IN TUMOR-BEARING MICE TREATED WITH LOW DOSE RADIATION

更新时间：2023-04-18

- AN EXPERIMENTAL STUDY ON THE ENHANCED MACROPHAGE (Mϕ) FUNCTION IN TUMOR-BEARING MICE TREATED WITH LOW DOSE RADIATION
- Journal of Radiation Research and Radiation Processing Vol. 16, Issue 4, Pages: 249-252(1998)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
- 作者简介：
- 基金信息：
  
  The National Natural Science Found ation of China(39270740)
- DOI：
  CLC：
扫描看全文
Zhang Ying, Sun Yimin, Li Xiuyi, et al. AN EXPERIMENTAL STUDY ON THE ENHANCED MACROPHAGE (Mϕ) FUNCTION IN TUMOR-BEARING MICE TREATED WITH LOW DOSE RADIATION. [J]. Journal of Radiation Research and Radiation Processing 16(4):249-252(1998)
DOI：

Zhang Ying, Sun Yimin, Li Xiuyi, et al. AN EXPERIMENTAL STUDY ON THE ENHANCED MACROPHAGE (Mϕ) FUNCTION IN TUMOR-BEARING MICE TREATED WITH LOW DOSE RADIATION. [J]. Journal of Radiation Research and Radiation Processing 16(4):249-252(1998) DOI：

摘要

采用C57BL/6J小鼠右后肢腓肠肌内接种Lewis肺癌细胞做为实验动物模型，在肿瘤接种后10d，给予荷瘤小鼠75mGyX射线全身照射，照射后18h处死。检测小鼠腹腔巨噬细胞（Mϕ）的肿瘤细胞静止效应，并采用原位杂交免疫组织化学方法测定腹腔Mϕ内肿瘤坏死因子α（TNFα）和白细胞介素1β（IL-1β）的mRNA转录水平。结果显示，受照射小鼠的Mϕ肿瘤静止效应由假照射组的52.9±8.1%增至79.9±4.2%（p<0.01）, Mϕ内TNFα和IL-1β的mRNA转录水平亦有明显提高。照射组的Mϕ内转录活性增高的阳性颗粒增多，染色加深，平均光密度值（MOD）由0.204±0.005, 0.216±0.015分别增至0.272±0.012和0.285±0.005,具有显著的差异（p<0.01）。提示小剂量辐射可显著增强荷瘤小鼠的Mϕ的功能。

Abstract

C57BL/6J mice implanted with Lewis lung carcinoma cells in the right thighs were used as an experimental animal model and whole body irradiation (WBI) with 75mGy X-rays was given at the 10th day after tumor implantation. We detected the cytostasis function of peritoneal Mϕ of the mice (with the method of ,3,H-TdR incorporation assay) and the levels of TNFα and IL-1β mRNA transcription (with the method of in situ hybridization immunohistochemistry) 18 hours after irradiation. The experiment showed that the cytostasis function of peritoneal Mϕ of irradiated mice was significantly increased compared with that of the sham-irradiated mice (irradiated mice: 79.9±4.2%, sham-irradiated mice: 52.9±8.1%,p,<,0.01). The levels of TNFα and IL-1β mRNA transcription were quantitatively measured with a scanning densitometry and the data showed that both the TNFα and IL-1β mRNA transcriptional levels of peritoneal Mϕ of irradiated mice were much higher than those of sham-irradiated mice (,p,<,0.01). The results mentioned above suggested that low dose radiation could markedly enhance the Mϕ function in tumor-bearing mice and the enhanced Mϕ function played an important role in the process of immunoenhancement and antitumor immune reaction induced by low dose radiation.

关键词

低剂量辐射巨噬细胞细胞静止效应肿瘤坏死因子α白细胞介素1 βmRNA转录

Keywords

Low dose radiationMacrophageCytostasisTNFαIL-1βmRNA transcription

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Interventional effect of multiple LDR on splenocyte apoptosis and immunity on twelve-week diabetic rats

Effect of calcium-binding protein S100A8 expression on early phase of radiation pulmonary fibrosis

Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation

HORMESIS OF LOW DOSE RADIATION ON THE EXPRESSION OF CYTOKINES IN UMBILICAL CORD BLOOD

ADVANCE OF STUDY ON HORMESIS OF LOW DOSE RADIATION

Related Author

No data

Related Institution

Department of Toxicology, School of Public Health, Jilin University

MH Radiobiology Research Unit, School of Public Health, Jilin University

Institute of Radiation Medicine, Academy of Military Medical Sciences

Institute of Modern Physics, Chinese Academy of Sciences

Graduate School of Chinese Academy of Sciences

⁰