THE DETECTION OF γ-RAY INDUCED DNA DOUBLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY MEMBRANE FILTRATION METHOD

Xia Shouxuan; Xu Huiying; Zhang Yangpei; Huang Sumin; Fan Guocai

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THE DETECTION OF γ-RAY INDUCED DNA DOUBLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY MEMBRANE FILTRATION METHOD

更新时间：2023-04-23

- THE DETECTION OF γ-RAY INDUCED DNA DOUBLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY MEMBRANE FILTRATION METHOD
- Journal of Radiation Research and Radiation Processing Vol. 2, Issue 1, Pages: 51-55(1984)
- 作者机构：
  
  1.军事医学科学院放射医学研究所
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Xia Shouxuan, Xu Huiying, Zhang Yangpei, et al. THE DETECTION OF γ-RAY INDUCED DNA DOUBLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY MEMBRANE FILTRATION METHOD. [J]. Journal of Radiation Research and Radiation Processing 2(1):51-55(1984)
DOI：

Xia Shouxuan, Xu Huiying, Zhang Yangpei, et al. THE DETECTION OF γ-RAY INDUCED DNA DOUBLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY MEMBRANE FILTRATION METHOD. [J]. Journal of Radiation Research and Radiation Processing 2(1):51-55(1984) DOI：

摘要

本文改进了Bradley和Kohn测定DNA双链断裂的微孔滤膜法，并用于检测γ射线引起的中国仓鼠卵巢细胞和小鼠骨髓细胞DNA的双链断裂，得到较好的剂量曲线，证明此法重复性好，灵敏度远较中性蔗糖密度梯度离心法高。同时也证明在上述细胞中辐射所致的DNA双链断裂是能够重接的。为DNA双链断裂能够重接的论点提供了又一个实验证据。

Abstract

The membrane filtration method of Bradley and Kohn was improved and used to detect the double strand breaks of DNA in irradiated Chinese hamster ovary cells and mouse bone marrow cells. The percentage of DNA retained on the membrane was inversely proportional to the radiation dose. The method had good reproducibility and was more simple and more sensitive than the classic neutral sucrose gradient centrifugation method. By this method the rejoining of double strand breaks could also be observed in both types of cells. The results provided an additional evidence that the double strand breaks could be repaired in mammalian cells.

关键词

DNA损伤DNA修复DNA双链断裂膜过滤法

Keywords

DNA DamageDNA RepairDNA Double strand breaksMembrane filtration method

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