STUDIES ON RADIATION- DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION

Zheng Xiulong; Zhao Fang; Ding Zhenying; Gao Jiangguo

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STUDIES ON RADIATION- DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION

更新时间：2023-04-23

- STUDIES ON RADIATION- DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION
- Journal of Radiation Research and Radiation Processing Vol. 3, Issue 1, Pages: 28-34(1985)
- 作者机构：
  
  1.上海第二军医大学
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Zheng Xiulong, Zhao Fang, Ding Zhenying, et al. STUDIES ON RADIATION- DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 3(1):28-34(1985)
DOI：

Zheng Xiulong, Zhao Fang, Ding Zhenying, et al. STUDIES ON RADIATION- DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 3(1):28-34(1985) DOI：

摘要

我们改进了能用于检测不分裂细胞DNA链断裂与重接的羟磷灰石离心-荧光法。证明了鼠血淋巴细胞能将γ-线引起的DNA断链重接。但受30 Gy损伤的细胞修复不完全，继续保温至8小时又发生断裂，同时细胞活力下降，再次肯定我们以前提出的观点：即重接DNA分子的再断裂是一种不可逆的生化变化，它可能是细胞死亡的预兆。

Abstract

A sensitive simple and rapid hydroxyapatite centrifugation-fluorometric method for detection of DNA strand breaks and its rejoining in nondividing cells was used and modified The circulating blood lymphocytes of rat were proved to be proficient in their ability to rejoin ,γ, induced strand breaks at 37℃. However, incomplete repairing was observed after irradiation with dosage of 30 Gy. If incubation was continued up to 8 hours, the rejoined DNA molecules were broken again and cell-death also increased at the same time. It was confirmed that the break of rejoined DNA molecules is an irreversible biochemical change which predicts the cell-death. This suggestion has been proposed in our foregoing paper.

关键词

羟磷灰石离心-荧光法大白鼠血淋巴细胞DNA链断裂DNA修复重接DNA再断裂

Keywords

Hydroxyapatite centrifugation-fluorometric methodRat blood lymp-hocytesDNA strand breakDNA rapairRejoined DNA break again

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