Using electronic beam radiation, the procedure for extraction and seperation of cytochrome b,5, reductase and the mechanism of reduction and oxidation reactions of this enzyme were described. Hydro-electron was produced when the solution was subjected to the action of electronic beam. From the spectrum of oxidized form of cytochrome b,5, reductase after reducing with hydro-electron, we discovered a new spectrum neither oxidized form nor reduced form, so we considered that it will be the existance of a middle form besides both oxidized and reduced form of this enzyme.