IMMOBILIZED GLUCOSE OXIDASE BY RADIATION INDUCED POLYMERIZATION OF HEMA AT LOW TEMPERATURE

Cao Jin; Su Zongxian

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IMMOBILIZED GLUCOSE OXIDASE BY RADIATION INDUCED POLYMERIZATION OF HEMA AT LOW TEMPERATURE

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- IMMOBILIZED GLUCOSE OXIDASE BY RADIATION INDUCED POLYMERIZATION OF HEMA AT LOW TEMPERATURE
- Journal of Radiation Research and Radiation Processing Vol. 6, Issue 1, Pages: 49-52(1988)
- 作者机构：
  
  1.华东化工学院，上海
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Cao Jin, Su Zongxian. IMMOBILIZED GLUCOSE OXIDASE BY RADIATION INDUCED POLYMERIZATION OF HEMA AT LOW TEMPERATURE. [J]. Journal of Radiation Research and Radiation Processing 6(1):49-52(1988)
DOI：

Cao Jin, Su Zongxian. IMMOBILIZED GLUCOSE OXIDASE BY RADIATION INDUCED POLYMERIZATION OF HEMA AT LOW TEMPERATURE. [J]. Journal of Radiation Research and Radiation Processing 6(1):49-52(1988) DOI：

摘要

本文研究在-78℃下，,60,Coγ辐射引发亲水性单体甲基丙烯酸β-羟乙酯（HEMA）聚合固定化葡萄糖氧化酶（GOD），实验结果证明，控制辐射总剂最不超过1×10,4,Gy，对葡萄糖氧化酶的活力损伤极微。载体HEMA浓度取为50%，包埋量是1.0 ml GOD/10 ml缓冲溶液（pH为5.6，0.06 mol/L磷酸缓冲液），可以得到既有弹性，又具有保存活力高的葡萄糖氧化酶固定化圆珠。辐照聚合HEMA温度越低，保存活力愈高。反应底物pH试验判明，固定化葡萄糖氧化酶的最适pH值与自然葡萄糖氧化酶的最适pH值一样，不过固定化葡萄糖氧化酶比自然酶更广泛适应于pH值的变化。固定化葡萄糖氧化酶动力学实验结果：米氏常数为k'm=1.42×10,-2,mol比自然酶km=1.0×10,-2,mol略高，说明了反应底物的扩散速度受到了一定的限制。活化能测定是13.7 kJ/mol。

Abstract

The immobilized glucose oxidase (GOD) by ,60,Co-γ induced polymerization of HEMA at -78℃ was studied. From the experiment results, it was found that the irradation dose until 1×10,4,Gy had not a significant effect on the native GOD activity. When the carrier (HEMA) concentration was 50%, entrapped amount 1.0 ml GOD/10 ml phosphoric acid buffer solution, the immobilized GOD had not only elastic, but also had high remaining activity. The native GOD was less sensitive fo pH value than the immobilized GOD, but both the proper pH values didn't change.,The kinetic reaction results showed, Michaelis constant k'm=1.42×10,-2,mol (native GOD km=1.0×10,-2,mol). This value indicated that diffuse velocity of substitue was restricted. The activation energies of the immobilized GOD were found to be 13.7 kJ/mol.

关键词

固定化酶包埋固定化葡萄糖氧化酶辐射引发聚合葡萄糖甲基丙烯酸β-羟乙酯

Keywords

Immobilized enzymeEntrapped immobilized methodGlucose oxidaseRadiation-induced polymerizationGlucoseβ-Hydroxyethyl Methacrylate(HEMA)

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