This article mainly deals with the observation of the growth of Escherichia coli, which was used as an experimental model on a nutritional medium, which isused as a food-substitute and pretreated with a certain dosage of ,60,Co irradiation. In the meantime, an Automicrobio system can be used to observe the variations of enzymic metabolism in contrast with the growth and generation-transference of the same bacterial strain on a non-irradiated medium.,It is shown that either in terms of microbic morphology, strain character, antigenic structure (Serum type) or of biochemical metabolic type. The various results are all in perfect conformity with those of the original strain. This demonstrates that though given a certain dosage of ,60,Co irradiation, the multi-nutritional medium does not affect the normal physiological metabolic function of the bacterial strain and induce mutagenesis in its further later generations.