THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H<sub>22</sub>) CELL NUCLEI

Jiang Yifeng; Zheng Xiulong

您当前的位置：

首页 >

文章列表页 >

THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI

更新时间：2023-04-23

- THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 3, Pages: 150-154,144(1990)
- 作者机构：
  
  1.第二军医大学，上海
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Jiang Yifeng, Zheng Xiulong. THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI. [J]. Journal of Radiation Research and Radiation Processing 8(3):150-154,144(1990)
DOI：

Jiang Yifeng, Zheng Xiulong. THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI. [J]. Journal of Radiation Research and Radiation Processing 8(3):150-154,144(1990) DOI：

摘要

本文采用DNA聚合酶,α,或／和,β,酶专一性抑制剂（NEM或／和d,2,TTP），观察了小鼠腹水型H,22,肝癌细胞核DNA聚合酶α、,β,在40 Gy γ线照后DNA修复合成中的作用。结果表明：在α酶被完全抑制条件下，照射组在37℃保温60、120 min后DNA修复合成均明显高于未受照组（P,<,0.05、P,<, 0.01）；当,β,酶被完全抑制条件下，照射组与未受照组在37℃保温30、60和120 min后DNA修复合成无明显差别（,P,>,0.05）；当α及,β,酶均被抑制时，照射组与未受照组DNA修复合成亦无明显差别（P,>, 0.05）。上述结果证明了在本实验条件下，,β,酶参与了受γ线照射后肝癌细胞核DNA的修复作用，而α酶则不参与。作者并对上述结果进行了讨论。

Abstract

The effects of DNA polymerase alpha and beta on DNA repair of the 40 Gy γ-rays irradiated H,22, cell nuclei, using specific inhibitor NEM or ddTTP to DNA polymerase alpha or beta respectively, were observed. The results showed that the rate of DNA repair synthesis of the 40 Gy γ-rays irradiated H,22, cell nuclei was much higher than that of the non-irradiated ones after 60,120 min incubation at 37℃(P,<,0.05,P,<,0.01)when DNA polymerase alpha was inhibited completely. There was no difference in the rate of DNA repair synthesis between the irradiated and the non-irradiated cell nuclei (P,>,0.05) when DNA polymerase beta was inhibited completely. And also, there was no difference between them (P,>,0.05) when both DNA polymerase alpha and beta were inhibited completely. These results indicate that under our experimental conditions, DNA polymerase beta, not alpha, is responsible for DNA repair of the γ-rays irradiated H,22, cell nuclei. The above results are discussed by the author considerably.

关键词

DNA聚合酶αDNA聚合酶βDNA修复合成NEMd2TTP

Keywords

DNA polymerase alphaDNA polymerase beta DNA repairNEMddTTP

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰

THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H22) CELL NUCLEI

DOI：

摘要

Abstract

关键词

Keywords

references

THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI