STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS

Guo Jinhui; Ha Hongfei; Zhang Yuhua

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STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS

更新时间：2023-04-23

- STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 3, Pages: 163-167(1990)
- 作者机构：
  
  1.北京大学技术物理系，北京
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Guo Jinhui, Ha Hongfei, Zhang Yuhua. STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS. [J]. Journal of Radiation Research and Radiation Processing 8(3):163-167(1990)
DOI：

Guo Jinhui, Ha Hongfei, Zhang Yuhua. STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS. [J]. Journal of Radiation Research and Radiation Processing 8(3):163-167(1990) DOI：

摘要

本文用γ辐射技术，在硅橡胶膜(Silastic）上接枝丙烯酰胺单体（AAM），用不同接枝率的Silastic-AAM膜通过叠氮化后对羊抗人IgG和出血热单克隆抗体（E,5,）进行了偶联固定化。同位素,125,碘标记方法对固定在Silastic-AAM膜上的羊抗人IgG量与接枝率的关系，以及抗体量与其免疫活性的关系进行了测定，得到了适于固定化的抗体偶联反应液最适浓度和最佳接枝率范围；用ELISA法对Silastic-AAM膜固定化的出血热（E,5,）单抗进行了免疫活性实验，得到了在某一实验条件下具有明显的阳性反应。结果表明，经适当改进，本法对临床诊断有一定应用价值。

Abstract

In this work films of silicone (silastic) were grafted by monomer acrylamide vis γ-radiation technology and then the ovine anti-human IgG, Epidemic hemorrhagic fever (EHF)-MCAb were immobilized on the silastic-AAM films with different grafting yields passthrough associate reactions. Measurements of relationships between grafting yields. contents of immobilized antibodies and immunoactivities for immobilized silastic-AAM films were performed by using ,125,I method ELISA method was used to measure the immunoactivities for the immobilized monoantibody. The results showed that the antibodies used can be immobilized on radiation-grafted silicone films and this immobilization method has its potential significance in clinical practice.

关键词

辐射接枝固定化125I标记ELISA法羊抗人IgG出血热单克隆抗体（E5）免疫活性

Keywords

Radiation graftingImmobilization125I laber methodELISA methodImmunoactivityOvine antihuman IgGEtidemic hemorrhagic fever monoantibody (E5)

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