STUDY ON THE REPAIR OF DNA SINGLE-STRAND BREAK IN CELLS AFTER RADIATION BY DOUBLE LABEI AKALINE EIUTION

He Jiewei; Michael A Bender

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STUDY ON THE REPAIR OF DNA SINGLE-STRAND BREAK IN CELLS AFTER RADIATION BY DOUBLE LABEI AKALINE EIUTION

更新时间：2023-04-23

- STUDY ON THE REPAIR OF DNA SINGLE-STRAND BREAK IN CELLS AFTER RADIATION BY DOUBLE LABEI AKALINE EIUTION
- Journal of Radiation Research and Radiation Processing Vol. 9, Issue 1, Pages: 39-43(1991)
- 作者机构：
  
  1.上海医科大学放射医学研究所
  2.Brookhaven National Lab. Long Island, New York
- 作者简介：
- 基金信息：
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He Jiewei, Michael A Bender. STUDY ON THE REPAIR OF DNA SINGLE-STRAND BREAK IN CELLS AFTER RADIATION BY DOUBLE LABEI AKALINE EIUTION. [J]. Journal of Radiation Research and Radiation Processing 9(1):39-43(1991)
DOI：

He Jiewei, Michael A Bender. STUDY ON THE REPAIR OF DNA SINGLE-STRAND BREAK IN CELLS AFTER RADIATION BY DOUBLE LABEI AKALINE EIUTION. [J]. Journal of Radiation Research and Radiation Processing 9(1):39-43(1991) DOI：

摘要

本文应用双标记碱性洗脱法对X射线照射引起的正常人纤母细胞、毛细血管扩张性共济失调症（AT）纤母细胞、AT肿瘤突变细胞DNA单链断裂及重接修复进行了研究。结果提示10 GyX射线照射对三种不同类型纤母细胞DNA的单链断裂与各自的不照射对照组比较有显著差别。而三种不同细胞株之间比较则无明显差别。细胞接受10 GyX射线照射后在37℃孵箱中孵育15、30和60 min可见三种细胞株DNA单链断裂都能立即进行重接修复，60 min内大部分已重接，细胞在10 Gy照射后加5×10,-4,mol/L aphidicolin／皿抑制聚合酶α，经37℃孵育60 min也有一定程度的修复。

Abstract

DNA single-strand break and rejoin were assayed in normal fibroblast ataxia telangiectasia (AT) and AT heterozygote cells by double label alkaline elution. The single-strand break in these three different lines of cells after 10 Gy X-radiation is about the same, but there is significantly different from the contral. After 37℃ incubation, the single-strand break of these three lines cells repaired accordingly with the time 15,30, 60 minutes. There are almost repaired within 60 minutes. The single-st-rand break in three different lines of cells are partly repaired after 37℃ incubation when 5×10,-4, mol/L aphidicolin was added.

关键词

电离辐射3H、14C双标记碱性洗脱DNA单链断裂与修复

Keywords

Radiation3H、14C double labelAlkalin elutionDNA single-strand break repair

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