STUDIES ON CHROMOSOMAL ABERRATIONS AND DOMINANT LETHAL MUTATIONS INDUCED BY X IRRADIATION IN GERM CELLS OF MALE MICE

Wang Xianli; Wang Mingdong; Wang Bin; Sun Shuqing

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STUDIES ON CHROMOSOMAL ABERRATIONS AND DOMINANT LETHAL MUTATIONS INDUCED BY X IRRADIATION IN GERM CELLS OF MALE MICE

更新时间：2023-04-23

- STUDIES ON CHROMOSOMAL ABERRATIONS AND DOMINANT LETHAL MUTATIONS INDUCED BY X IRRADIATION IN GERM CELLS OF MALE MICE
- Journal of Radiation Research and Radiation Processing Vol. 10, Issue 2, Pages: 97-101(1992)
- 作者机构：
  
  1.白求恩医科大学放射医学研究所，长春
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Wang Xianli, Wang Mingdong, Wang Bin, et al. STUDIES ON CHROMOSOMAL ABERRATIONS AND DOMINANT LETHAL MUTATIONS INDUCED BY X IRRADIATION IN GERM CELLS OF MALE MICE. [J]. Journal of Radiation Research and Radiation Processing 10(2):97-101(1992)
DOI：

Wang Xianli, Wang Mingdong, Wang Bin, et al. STUDIES ON CHROMOSOMAL ABERRATIONS AND DOMINANT LETHAL MUTATIONS INDUCED BY X IRRADIATION IN GERM CELLS OF MALE MICE. [J]. Journal of Radiation Research and Radiation Processing 10(2):97-101(1992) DOI：

摘要

2.0 Gy X射线照射雄鼠不同发育阶段的生殖细胞与超数排卵处理的正常雌鼠交配，制备受精卵染色体标本。结果表明：雄性原核染色体畸变量以精细胞>成熟精子>精母细胞>精原细胞的顺序发生，其中前二者与自身正常对照比较，差异非常显著（P<0.01），与国外有关报道相一致。照后170 d的精原细胞易位，明显高于正常对照，而且与照后140 d观察的显性致死突变明显相关。实验证实用受精卵技术检测减数分裂后染色体损伤是良好的实验手段，而观察精原干细胞易位，用精母细胞实验更理想。

Abstract

After male mice irradiated by 2Gy X rays mated to normal virginal females superovulated with PMSG and HCG, pronuclei chromosome spreading of first-cleavage embryos were prepared and chromosomal aberrations of paternal pronuclei were observed, The results showed that the frequency of chromosomal aberrations was highest irradiated at spermatid stage among different stages of spermatogenesis. The sequency of radiosensitivity in spermatogenesis was as follows: spermatid> mature sperm> spermatocyte> spermatogonium and stem spermatogonium. The frequencies of paternal chromosomal aberrations resulted from irradiation at spermatids and mature sperms were significantly higher than that in control.,The reciprocal translocations of stem spermatogonia induced by 2Gy X rays in those male mice were also examined in the preparations of diakinesis-metaphase Ⅰ. The freqency of reciprocal translocations were 0.0429 per cell and significantly higher than that in control. The proportion of unbalanced gametes, resulting in lethal embryos after fertilization, was 0.02145 to be predicted. At the same time, the dominant lethality induced by X rays in stem spermatogonia was measured, being 0.0371. The frequency of dead fetuses in irradiation group was about twice as in control. The regression analysis was found that the reciprocal translocations was markedly related to the dominant lethality.

关键词

X射线生殖细胞雄性原核染色体畸变小鼠显性致死突变

Keywords

X rayGerm cellPaternal pronucleusChromosomal aberrationReciprocal translocationDominant lethality

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