THE EFFECT OF γ-RAY IRRADIATION ON PLASMID pBR 322 DNA

Zhou Lijun; Fang Yunzhong; Zhang Yangpei; Wei Kang

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THE EFFECT OF γ-RAY IRRADIATION ON PLASMID pBR 322 DNA

更新时间：2023-04-23

- THE EFFECT OF γ-RAY IRRADIATION ON PLASMID pBR 322 DNA
- Journal of Radiation Research and Radiation Processing Vol. 11, Issue 1, Pages: 28-30,57(1993)
- 作者机构：
  
  1.军事医学科学院放射医学研究所，北京　100850
- 作者简介：
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Zhou Lijun, Fang Yunzhong, Zhang Yangpei, et al. THE EFFECT OF γ-RAY IRRADIATION ON PLASMID pBR 322 DNA. [J]. Journal of Radiation Research and Radiation Processing 11(1):28-30,57(1993)
DOI：

Zhou Lijun, Fang Yunzhong, Zhang Yangpei, et al. THE EFFECT OF γ-RAY IRRADIATION ON PLASMID pBR 322 DNA. [J]. Journal of Radiation Research and Radiation Processing 11(1):28-30,57(1993) DOI：

摘要

采用琼脂糖凝胶电泳及激光密度扫描仪和积分分析，观察了质粒pBR 322 DNA的γ辐射效应。发现随照射剂量的增加，超螺旋质粒所占比例逐渐下降，而开环质粒所占比例相应升高；运用OH自由基清除剂甲酸钠进一步观察到清除OH自由基后，质粒pBR 322的辐射效应大为减轻。结果提示其损伤主要是通过水的辐解产物OH自由基的间接作用而引起的。

Abstract

Using the agarose gel electrophoresis in combination with laser densitometer and recording integrator, the effect of γ-ray irradiation on plasmid pBR 322 was studied. It was found that the amount of supercoiled (SC) DNA decreased with the increase of irradiation dose, whereas the nicked circular (OC) DNA increased. Formate, a well known hydroxyl free radical scavenger, could decreased the radiosensitivity of the pBR 322 DNA, indicating that the effect of the ,60,Co irradiation on plasmid was mainly attributable to the indirect action of radiation, Which is resulted from OH radical produced by the radiolysis of water, giving rise to single strand break of DNA.

关键词

γ辐射DNA损伤质粒pBR 322清除剂

Keywords

γ-irradiationDNA damagePlasmid pBR 322Free radical scavenger

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