THE EFFECTS OF RADIATION ON T-LYMPHOCYTES IN PERIPHERAL BLOOD AND CFU-TL

Cao Xia; Wang Tao; Li Yanyi

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THE EFFECTS OF RADIATION ON T-LYMPHOCYTES IN PERIPHERAL BLOOD AND CFU-TL

更新时间：2023-04-23

- THE EFFECTS OF RADIATION ON T-LYMPHOCYTES IN PERIPHERAL BLOOD AND CFU-TL
- Journal of Radiation Research and Radiation Processing Vol. 11, Issue 3, Pages: 189-192(1993)
- 作者机构：
  
  1.苏州医学院放射医学系，苏州　215007
- 作者简介：
- 基金信息：
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Cao Xia, Wang Tao, Li Yanyi. THE EFFECTS OF RADIATION ON T-LYMPHOCYTES IN PERIPHERAL BLOOD AND CFU-TL. [J]. Journal of Radiation Research and Radiation Processing 11(3):189-192(1993)
DOI：

Cao Xia, Wang Tao, Li Yanyi. THE EFFECTS OF RADIATION ON T-LYMPHOCYTES IN PERIPHERAL BLOOD AND CFU-TL. [J]. Journal of Radiation Research and Radiation Processing 11(3):189-192(1993) DOI：

摘要

利用甲基纤维素微量培养和,3,H-TdR掺入法，研究了T淋巴细胞增殖动力学、辐射敏感性及T淋巴细胞集落形成单位（CFU-TL）。结果表明，外周血单个核细胞在一定浓度范围内，CFU-TL形成数随种植细胞数而增加，呈良好线性关系；培养12 d的集落细胞在PHA-CM刺激下仍具有,3,H-TdR掺入能力，但计数下降，表明培养后的集落细胞DNA合成能力减弱；γ射线辐照后，随剂量增加，集落数和,3,H-TdR掺入数迅速降低，CFU-TL对辐射很敏感，D,0,=2.3 Gy。

Abstract

The kinetics, metabolism and radiosensitivity of T-lymphocytes in peripheral blood and colony forming unit-T lymphocyte (CFU-TL) were studied with technique of tissue culture with methycellulose and ,3,H-TdR incorporation. The linear relationship was observed between the number of CFU-TL and the planted mononucleus cells at a certain concentration of cells. The number of colonies increased with planted cells in the range of (0.5—1.5)×10,5,. It was found that in the cells cultured for 12 days,3,H—TdR incorporation still can be achieved but incorporation was lower than that in fresh separated mononucleus cells, which suggests decreased in the exposed cells. After γ-irradiation, both the number of colonies and CPM of ,3,H-TdR incorporation decreased rapidly. The result indicates that CFU-TL is more sensitive, D,0,=2.3 Gy.

关键词

T淋巴细胞集落形成单位（CFU-TI）增殖特性放射敏感性生物辐射效应

Keywords

T-lymphocyte colony forming unit (CFU-TL)Proliferation characteristicRadiosensitivityBiological radiation effects

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Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology

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苏州大学医学部放射医学与防护学院/放射医学及交叉学科研究院

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