IMMOBILIZATION OF BAKER'S YEAST BY RADIATION-INDUCED POLYMERIZATION OF ACRYLAMIDE

Su Zongxian; Cao Jin; Wang Fujun; Sui Peng

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IMMOBILIZATION OF BAKER'S YEAST BY RADIATION-INDUCED POLYMERIZATION OF ACRYLAMIDE

更新时间：2023-04-26

- IMMOBILIZATION OF BAKER'S YEAST BY RADIATION-INDUCED POLYMERIZATION OF ACRYLAMIDE
- Journal of Radiation Research and Radiation Processing Vol. 4, Issue 2, Pages: 49-53(1986)
- 作者机构：
  
  1.华东化工学院
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Su Zongxian, Cao Jin, Wang Fujun, et al. IMMOBILIZATION OF BAKER'S YEAST BY RADIATION-INDUCED POLYMERIZATION OF ACRYLAMIDE. [J]. Journal of Radiation Research and Radiation Processing 4(2):49-53(1986)
DOI：

Su Zongxian, Cao Jin, Wang Fujun, et al. IMMOBILIZATION OF BAKER'S YEAST BY RADIATION-INDUCED POLYMERIZATION OF ACRYLAMIDE. [J]. Journal of Radiation Research and Radiation Processing 4(2):49-53(1986) DOI：

摘要

本文研究用,60,Co辐射聚合丙烯酰胺，包埋具有转化酶活力的酵母细胞的方法。丙烯酰胺作为载体，NN'-甲撑双丙烯酰胺作为交联剂。探讨了固定化的一些条件，如辐照剂量、聚合温度、酵母和交联剂的用量对酶活力的影响。试验发现，辐照剂量高达2×10,6,rad，对酶活力没有影响。由最适条件所制得的固定化细胞既有弹性又有硬度，并且显示较高的活力回收，重复10次反应后酶活力保持不变。

Abstract

The method for entrapment of Baker's yeast cell with invertase activity by ,60,Co radiation-induced polymerization of acrylaminde has been studied.,Acrylaminde was used as a carrier and N, N'-methylene bisacrylamide as a cross-linking reagent. The influence of immobilized conditions (such as irradiation dose, polymerization temperature, amount of yeast and cross-linking reagent) on the enzymatic activity has been investigated. From the experiments it was found that the irradiation dose until 2×10,6,rad has not a significant effect on the enzymatic activity. The immobilized cell which was prepared according to the optimum conditions was not only elastic but also hard and exhibited higher activity recovery. After the repeated use 10 tims of batch reaction the enzymatic activity didn't change.

关键词

辐射聚合丙烯酰胺转化酶蔗糖

Keywords

Radiation-induced polymerizationAcrylamideInvertaseSucrose

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