RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY

Meng Ziqiang

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RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY

更新时间：2023-04-26

- RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY
- Journal of Radiation Research and Radiation Processing Vol. 5, Issue 4, Pages: 14-18(1987)
- 作者机构：
  
  1.山西大学
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Meng Ziqiang. RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY. [J]. Journal of Radiation Research and Radiation Processing 5(4):14-18(1987)
DOI：

Meng Ziqiang. RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY. [J]. Journal of Radiation Research and Radiation Processing 5(4):14-18(1987) DOI：

摘要

本文运用羟基磷灰石柱层析法对,60,Co γ线引起的中国田鼠肺细胞（CHL）和615小鼠离体脾细胞DNA单链断裂和重接进行了研究。在1～10 Gy剂量范围内，两种细胞DNA单链断裂的程度均与剂量呈线性关系。两种细胞每单位剂量诱发的DNA单链断裂的数量是相似的。CHL细胞DNA单链断裂能迅速进行重接，其重接速率和程度与剂量呈负相关。同时，单链重接与培养条件也密切相关，CHL细胞在无血清培养液中比在含小牛血清培养液中其DNA断链重接速率慢、程度低，而离体的小鼠脾细胞DNA断链在无血清培养液中甚至未见重接修复。

Abstract

The ,60,Co γ-ray induced single strand breaks of DNA and their rejoining in cultured chinese hamster lung (CHL) cells and mouse spleen cells isolated freshly from mice were detected by hydroxylapatite chromatography. The method is based on the observation that the rate of denaturation of DNA to single strands in alkali is increased after irradiation, the interpretation being that this effect is due to DNA strand breaks. The percentage of labeled DNA that remained double strand after 30 min alkali treatment was inversely proportional to the radiation dose within the range of 1-10Gy for the two kinds of cells.,The DNA strand breaks in unirradiated spleen cells was more than in unirradiated CHL cells, but the yield of breaks induced per 0.01Gy was found to be similar in the spleen cells and CHL cells. In contrast with these findings, most DNA strand breaks in the CHL cells were rejoined within 30min, the breaks were rejoined faster in the cells incubated in medium with 20% new born calf serum after irradiation than in medium without any serum. The repair of damaged DNA could not be detected in the spleen cells cultured in RPMI 1640 medium without any serum.

关键词

羟基磷灰石柱层析法DNA链断裂DNA修复CHL细胞小鼠脾细胞

Keywords

Hydroxylapatite chromatographyDNA strand breaksDNA repairCHL cellsMouse spleen cells

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