THE ROLES OF DNA POLYMERASE β IN HEPATOMA CELLS DNA REPAIR SYNTHESIS AFTER γ-RAY IRRADIATION

Cai Jianming; Zheng Xiulong; Gao Jianguo; Li Bailong; Yang Lixi; Yang Rujun; Luo Chengji; Cheng Tianmin

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THE ROLES OF DNA POLYMERASE β IN HEPATOMA CELLS DNA REPAIR SYNTHESIS AFTER γ-RAY IRRADIATION

更新时间：2023-04-26

- THE ROLES OF DNA POLYMERASE β IN HEPATOMA CELLS DNA REPAIR SYNTHESIS AFTER γ-RAY IRRADIATION
- Journal of Radiation Research and Radiation Processing Vol. 14, Issue 2, Pages: 96-100(1996)
- 作者机构：
  
  1.第二军医大学放射医学研究所，上海 200433
  2.第三军医大学防原教研室，重庆 630038
- 作者简介：
- 基金信息：
- DOI：
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Cai Jianming, Zheng Xiulong, Gao Jianguo, et al. THE ROLES OF DNA POLYMERASE β IN HEPATOMA CELLS DNA REPAIR SYNTHESIS AFTER γ-RAY IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 14(2):96-100(1996)
DOI：

Cai Jianming, Zheng Xiulong, Gao Jianguo, et al. THE ROLES OF DNA POLYMERASE β IN HEPATOMA CELLS DNA REPAIR SYNTHESIS AFTER γ-RAY IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 14(2):96-100(1996) DOI：

摘要

利用第二军医大学裸鼠移植性肝癌细胞（SMMC-LTNM）和不同的DNA聚合酶选择性抑制剂NEM与d,2,TTP，探讨了肿瘤细胞核中DNA聚合酶,β,参与γ射线辐射损伤DNA修复的作用特点。结果表明，该酶参与真核细胞γ射线辐照损伤的DNA修复合成，是一种重要的DNA修复酶；与正常肝细胞相比，SMMC-LTNM肝癌细胞DNA聚合酶,β,的DNA修复合成反应速度快，修复能力强。提示某些肿瘤细胞中该酶的DNA辐射损伤修复合成功能比正常细胞的大。

Abstract

The roles and characteristics of DNA polymerase ,β, of hepatoma cells in participating in DNA repair synthesis induced by ,60,Co γ-rays irradiation were studied. Transplanted SMMC-LTNM hepatoma in mice was used as a tumor cells model. NEM and d,2,TTP were used as selective inhibitors to DNA polymerase α and ,β, respectively. It was showed that the polymerase ,β, could take part in repair synthesis of DNA damaged by γ-rays. The increment of ,3,H-TTP incorporation into DNA of SMMC-LTNM hepatoma cells nuclei was higher than that of normal hepatocyte nuclei (P,<,0.01). The DNA repair synthesis induced by DNA polymerase ,β, reacted more fast in hepatoma cells nuclei than in hepatocytes nuclei too. These results indicated that the DNA repair synthesis of polymerase ,β, in some tumor cells might be stronger than normal cells.

关键词

脱氧核糖核酸聚合酶β脱氧核糖核酸修复SMMC-LTNM肝癌γ射线

Keywords

DNA polymerase βDNA repairSMMC-LTNM hepatomaγ-radiation

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