THE STUDY ON CELL GLUTATHIONE LEVELS AND RADIOSENSITIZATION

Jin Yizun; Ding Li; Yan Minfen

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THE STUDY ON CELL GLUTATHIONE LEVELS AND RADIOSENSITIZATION

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- THE STUDY ON CELL GLUTATHIONE LEVELS AND RADIOSENSITIZATION
- Journal of Radiation Research and Radiation Processing Vol. 14, Issue 3, Pages: 172-176(1996)
- 作者机构：
  
  上海医科大学放射医学研究所　上海　200032
- 作者简介：
- 基金信息：
  
  THe project was supported by The National Science Foundation of China(39130050)
- DOI：
  CLC：
- Published：1996-08，
  
  Received：28 March 1995，
  
  Revised：29 February 1996，
- 稿件说明：
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Jin Yizun, Ding Li, Yan Minfen. THE STUDY ON CELL GLUTATHIONE LEVELS AND RADIOSENSITIZATION. [J]. Journal of Radiation Research and Radiation Processing, 1996,14(3):172-176.
DOI：

Jin Yizun, Ding Li, Yan Minfen. THE STUDY ON CELL GLUTATHIONE LEVELS AND RADIOSENSITIZATION. [J]. Journal of Radiation Research and Radiation Processing, 1996,14(3):172-176. DOI：

摘要

谷胱甘肽（GSH）是一种内源性放射防护物质，在正常状况下大部分以还原型存在于细胞和组织中。本研究结果表明，通过外源性化学修饰剂丁胱亚磺酰亚胺（BSO），可有效地抑制细胞内GSH的生物合成，从而使内源性GSH含量下降，在1 mmol/L BSO作用后24 h，可使原有的GSH含量从4.67684 ×10

-12

mmol/cell下降至0.05092×10

-12

mmol/cell，为对照组的1.09%。BSO的抑制作用与其浓度和作用时间长短有关：0.1 mmol/L BSO作用2h后，GSH含量为2.63448×10

-12

mmol/cell，而在作用48 h后，GSH含量为0.09277×10

-12

mmol/cell。即BSO在0.1 mmol/L浓度时，作用48 h后细胞内GSH下降为对照组的1.98%。细胞在GSH含量降低的情况下，进行电离辐射，通过对细胞放射增敏比（SER）的观察表明，GSH下降时SER值增大，说明减少GSH的含量，可增加细胞对射线的敏感性。这对放射生物学和临床放射肿瘤学的理论与实践均有一定意义。

Abstract

The role of intracellar glutathione (GSH) in radiation protection has been noticed in recent years. In this study

we found that the level of intracellar glutathione and radiosensitization had a certain relationship. It was recognized that the higher GSH level

the lower radiosensitization. The GSH level in V 79-379 A

Y 79

SPC-A-1

SGC-7901 and So-Rb 50 cells were showed in this experiment

s by Tietze method. The GSH level which was depleted by BSO was assessed. The radiosensitization was determined by the standard colony formation assay

IN VITRO

. The results showed that SER (Sensitization Enhancement Ratio) for V 79-379 A cell was increased with GSH depletion. The SER for BSO 0.1

0.5 and 1.0 mmol/L were 1.16

1.34 and 1.50

respectively. Same results for Y 79 cells were also observed that the SER for BSO 0.01 and 0.05 mmol/L were 1.21 and 1.36 respedively. On the other hand

the exposure time of BSO also influenced GSH level and SER. These results suggested that the level of glutathione in cell and radiosensitization has a close relationship.

关键词

谷胱甘肽丁胱亚磺酰亚胺放射敏感性

Keywords

Glutathione (GSH)Buthione sulfoximine (BSO)Radiosensization

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