ESR STUDY ON RADIATION PROTECTION OF HISTONE H3 BY HYDROXYCINNAMIC ACID DERIVATIVES

Lin Weizhen; Tu Tiecheng; Dong Jirong; Yao Side; Zhang Jiashan; Lin Niayun

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ESR STUDY ON RADIATION PROTECTION OF HISTONE H3 BY HYDROXYCINNAMIC ACID DERIVATIVES

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- ESR STUDY ON RADIATION PROTECTION OF HISTONE H3 BY HYDROXYCINNAMIC ACID DERIVATIVES
- Journal of Radiation Research and Radiation Processing Vol. 15, Issue 4, Pages: 210-216(1997)
- 作者机构：
  
  中国科学院辐射化学研究开放实验室　上海原子核研究所　上海　201800
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1997-11，
  
  Received：27 November 1996，
  
  Revised：08 July 1996，
- 稿件说明：
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Lin Weizhen, Tu Tiecheng, Dong Jirong, et al. ESR STUDY ON RADIATION PROTECTION OF HISTONE H3 BY HYDROXYCINNAMIC ACID DERIVATIVES. [J]. Journal of Radiation Research and Radiation Processing, 1997,15(4):210-216.
DOI：

Lin Weizhen, Tu Tiecheng, Dong Jirong, et al. ESR STUDY ON RADIATION PROTECTION OF HISTONE H3 BY HYDROXYCINNAMIC ACID DERIVATIVES. [J]. Journal of Radiation Research and Radiation Processing, 1997,15(4):210-216. DOI：

摘要

应用ESR波谱模拟解析法，研究了77K γ辐射引发羟基肉桂酸衍生物与组蛋白H3的二元分子复合体系两组分间的电子转移过程。在解析二元复合体系ESR波谱的基础上，计算了二元分子复合体系两组分摩尔比不同的一系列复合体系的自旋转移比Rsc。研究发现Rsc与复合体系的摩尔比呈良好的线性关系，这表明羟基肉桂酸衍生物具有较强的夺取蛋白质肽链酰胺羰基阴离子自由基电子和修复蛋白质辐射损伤的能力，这与用羟基肉桂酸衍生物通过电荷转移对生物大分子辐射损伤的保护、修复机理的前期研究结果相符。在研究了辐射引发的组蛋白H3-咖啡酸分子复合体系自旋转移比的pH效应基础上，阐明了分子复合体系内蛋白质肽链酰胺羰基与咖啡酸间的氢键是实现体系内分子间电子转移的主要通道。

Abstract

Radiation induced electron transfer between histone H3 and some hydroxycinnamic acid derivatives within binary molecular aggregates has been in vestigated by means of resolution and simulation of ESR spectra from those aggregates γ-irradiated and recorded at 77K

respectively. The excellent linear relationships

which were made on the basis of simulation of composite ESR spectra relevant to those binary aggregates with various molar ratios of the two components

between spin transfer ratios(Rsc) and molar ratios (r) of the aggregates indicate that those hydroxycinnamic acid derivatives have effective potential for repair of the radiation damaged protein by abstracting electrons on peptide carbonyl radical anions in protein. These results are in good accord with our previous studies on radiation protection of DNA and proteins via charge transfer from the damaged biomolecules to hydroxycinnamic acid derivatives. The hydrogen bond formed between peptide carbonyl in protein and phenolic hydroxyl of hydroxycinnamic acids within the aggregates has been illustrated as the major pathway for electron transfer from H3 to caffeic acid hased upon pH dependence on the linear relationship between Rsc and r of irradiated histone H3-caffeic acid aggregates.

关键词

组蛋白H3咖啡酸芥子酸电子转移酰胺羰基阴离子自由基酚羟基自由基

Keywords

Histone H3Caffeic acidSinapic acidEle ctron transferPeptide carbonyl radical anionPhenolic hydroxyl radical

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