DYNAMIC CHANGES OF THE EARLY PROTEIN SYNTHESIS IN MURINE IMMUNE CELLS AFTER LOW DOSE RADIATION

Chen Shali; Liu Shuzheng

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DYNAMIC CHANGES OF THE EARLY PROTEIN SYNTHESIS IN MURINE IMMUNE CELLS AFTER LOW DOSE RADIATION

更新时间：2024-11-05

- DYNAMIC CHANGES OF THE EARLY PROTEIN SYNTHESIS IN MURINE IMMUNE CELLS AFTER LOW DOSE RADIATION
- Journal of Radiation Research and Radiation Processing Vol. 15, Issue 4, Pages: 238-241(1997)
- 作者机构：
  
  白求恩医科大学卫生部放射生物实验室　长春　130021
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1997-11，
  
  Received：12 August 1996，
  
  Revised：21 October 1996，
- 稿件说明：
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Chen Shali, Liu Shuzheng. DYNAMIC CHANGES OF THE EARLY PROTEIN SYNTHESIS IN MURINE IMMUNE CELLS AFTER LOW DOSE RADIATION. [J]. Journal of Radiation Research and Radiation Processing, 1997,15(4):238-241.
DOI：

Chen Shali, Liu Shuzheng. DYNAMIC CHANGES OF THE EARLY PROTEIN SYNTHESIS IN MURINE IMMUNE CELLS AFTER LOW DOSE RADIATION. [J]. Journal of Radiation Research and Radiation Processing, 1997,15(4):238-241. DOI：

摘要

采用

H-Leu掺入示踪观察75 mQyX射线全身照射对小鼠免疫细胞蛋白质早期合成的影响。结果表明，小剂量X射线全身照射后小鼠胸腺细胞蛋白质合成开始增加，6～8h达到峰值，随后逐渐下降。SDS-PAGE及光密度扫描结果表明，照射后小鼠胸腺及脾细胞蛋白质合成增加，同一细胞中不同分子量的蛋白质各有其消长特点，不同细胞间合成增加的蛋白质分子量有所不同。这些早期变化的蛋白分子参与复杂的细胞功能调节，可能与辐射兴奋效应有关。

Abstract

It was shown that there was a marked increase in protein synthesis of thymocytes that were metabolically labelled with

H-Leu for 4

8 and 12 hous in low dose irradiated mice showing 33.26%

51.48%

51.54% and 34.98% increase respectively at different time intervals of incubation when the thymic and splenic cells were sampled 4 hours after whole body irradiation (WBI) with 75 mGy X-rays. The results suggest that there is an increase in protein synthesis with its peak at 6~8 hours after radiation. Changes in protein synthesis of immune cells in mice 4 hours after radiation and incubated for 4~12 h were observed with SDS-PAGE followed by densitometrical scanning. It is revealed that 28 kD protein synthesis was increased gradually within 12 hours of incubation and 43 kD protein synthesis was increased in the thymocytes rapidly reaching a maximum 2 hours after incuba

tion. It was also exhibited that the synthesis of 43 kD protein and 32 kD protein was increased in the splenocytes 2 hours after incubation. These findings may have implications in the mechanism of immunoenhancement and adaptive response induced by low dose radiation.

关键词

小剂量辐射免疫细胞蛋白质合成动力学

Keywords

Low dose radiationImmune cellProteinSynthesis

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