The study of heavy ion beams as a new irradiation resource on seeds of linseed (,Linum usitatissmum L.,) was reported. After irradiated by ,12,C ,6+, heavy ions (with irradiation dose: 6×108 cm,-2, 1.8×10,9, cm,-2, and 3.6×10,9, cm,-2, respectively), the biological characters, agricultural traits, pollens and polymorphic DNA of sample was studied in ,Linum usitatissmum L.,. The results showed that 6×10,8, cm,-2, of ,12,C ,6+, could promote the linseed germination rate, plant height and pollen viability. The 1000-seed weight and the oil content of seed irradiated by ,12,C,6+, heavy ions was increased with augment of irradiation dose respectively. It has been found that 3.6×10,9, cm,-2, is the most effective dose for increment both of seed weight and oil content with 16.5% and 19.9%, respectively. Cytological observation reveals that the highest dose (3.6×10,9, cm,-2,) can also induce alteration of pollen shape of linseed. Furthermore, RAPD(Randomly amplified polymorphic DNAs) analysis shows that 99 DNA fragments are amplificated by 14 primers and the polymorphic ratio is 52.5%.