Lentinula edodes, were implanted with various nitrogen ions implantation dosage but the same energy in this study. The variations of growth rate of mycelium, mycelial biomass and the contents of lentinan(LNT) under various nitrogen ions implantation dosage were investigated. Three curves of implantation all take a "saddle shape" with increasing N,+, ion implanted doses. The growth rate of mycelium is high under low dosage of N,+, implantation, but with the increment of dosage, the growth rate shows downtrend then began rising when the dosage reaching 1.5×10,16, cm,-2,. The mycelia biomass and the contents of LNT rise firstly and then fall with the increment of dosage, and they both reach maximum at the dosage of 4×10,15, cm,-2,. Some fluctuation exists in the process, so they both reach another peak at the dosage of 1.5×10,16, cm,-2,. During the liquid culture of ,Lentinula edodes, the variation of mycelial biomass, gross weight of shake-flask, pH and the contents of lentinan(LNT) has been studied. The consistency between the variation of mycelia biomass and gross weight of flask during the liquid culture has also been confirmed. This result can be applied to the study on fermentation before and mutation. Thus a conclusion may be drawn that it is an effective way of mutation breeding by N,+, ion implantation.