Blakeslea trispora,(−) was used as experimental model, and the effects of N,+, ions implantation on the activities of their catalase (CAT), peroxidase (POD), total antioxidase (T-AOC), superoxide dismutase (SOD) and the function of scavenging free-radicals (O,2,–•, and ·OH ) were investigated. The results show that CAT activities are lower than that of control groups. With the increasing of N,+, ion implantation POD, SOD activities and the function of scavenging O,2,–•, and ·OH decrease at the beginning, and increase lately. At the dose of 6.0×10,15, cm,-2, T-AOC activity is the lowest and at the dose of 1.2×10,15, cm,-2, its activity becomes the highest. It can be considered that the changes of T-AOC, POD, SOD CAT activities and cell scavenging O,2,–•, and ·OH activities have some relationship with the "saddle shape" survival rate curve in the ion implantation breeding.