To explore the biological effects of microwave combined with γ-rays on apoptosis, mitochondrion membrane potential(MMP), Ca,2+,and Ca,2+,-Mg,2+,-ATPase in rat cultured gliacytes, primarily cultured gliacytes were randomly divided into the control group(C), the microwave group(M), the γ-rays group(I) and the combined group (IM). The M and IM groups were exposed to 4 mW/cm,2, microwave 2h/d for 3 days. The I and IM groups were given 5 Gy ,60,Co γ-rays on the forth day. Ca,2+,-Mg,2+,-ATPase was detected by kit. Apoptosis, MMP and Ca,2+, were detected by flow cytometry(FCM). The results show that the ratio of apoptosis of the I and IM groups were significantly increased, and the contents of Ca,2+, in the cytoplasm of M, I and IM group cells were all significantly higher meanwhile Ca,2+,-Mg,2+,-ATPase activity was lower than that of the control group; but MMP had no obvious changes in the four groups. Under the experimental conditions, the microwave radiation did not induce evident changes in MMP. However, the content of Ca,2+, in the cytoplasm increased significantly and Ca,2+,-Mg,2+,-ATPase decreased obviously. No synergic effects were observed in the treatment with microwave before exposure to γ-rays.