In order to compare the radiation sensitizing effect of artemisinin and artemether to human nasopharyngeal carcinoma CNE-1 cell, the human nasopharyngeal carcinoma CNE-1 cell in index vegetal period was selected as experimental sample, and the cells clone-forming method was used to test growth inhibition of artemisininand and artemether to the CNE-1 cell, to estimate the difference of inhibitory effect between the two drugs, and to determine suitable drug concentration. The experimental samples were divided into control group, simple drug group, irradiated group and union treatment group with human nasopharyngeal carcinoma cells. The simple drug and union treatment group were also divided into artemisinin and artemether group, respectively. The exposed doses were set up as 0, 2, 4, 6, and 8 Gy. The cell survival curve was fitted by an multi-target one-shot mathematic model and taking the radiation sensitizing enhancement ratio/SER (SER) equal to the dosage ratio of irradiated group and union treatment group when the same biological effect was achieved. It has been found that both drugs can enhance the inhibitory effect with the increment of medicine concentration. The radiation sensitizing enhancement ratio/SER (SER) has been determined to be 1.272 and 1.481for artemisinin and artemether, respectively, which suggests that artemether has stronger radiation sensitizing effect compared with artemisinin in human nasopharyngeal carcinoma CNE-1 cells.