Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究

于雷; 孙世龙; 方芳; 刘永哲; 巩宏伟; 石磊; 鞠桂芝

您当前的位置：

首页 >

文章列表页 >

Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究

研究简报 | 更新时间：2023-03-30

- Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究
- Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays
- 辐射研究与辐射工艺学报 2010年28卷第3期页码：190-192
- 作者机构：
  
  1.吉林大学公共卫生学院　长春　130021
- 作者简介：
  
  于雷，男，1977年9月出生，2006年于吉林大学获硕士学位，讲师，主要从事辐射肿瘤学研究
  石磊，E-mail: shilei0242002@yahoo.com
- 基金信息：
  
  国家自然科学基金项目(30670630);吉林大学研究生创新基金项目(20091032)
- DOI：
  中图分类号：
扫描看全文
于雷, 孙世龙, 方芳, 等. Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究[J]. 辐射研究与辐射工艺学报, 2010,28(3):190-192.

YU Lei, SUN Shilong, FANG Fang, et al. Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays[J]. Journal of Radiation Research and Radiation Processing, 2010,28(3):190-192.
于雷, 孙世龙, 方芳, 等. Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究[J]. 辐射研究与辐射工艺学报, 2010,28(3):190-192. DOI：

YU Lei, SUN Shilong, FANG Fang, et al. Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays[J]. Journal of Radiation Research and Radiation Processing, 2010,28(3):190-192. DOI：

摘要

为探讨kras基因与辐射诱发小鼠胸腺淋巴瘤的关系，先采用X射线照射BALB/c小鼠建立胸腺淋巴瘤模型，取胸腺淋巴瘤和正常胸腺，分别提取总RNA、合成cDNA和提取总蛋白，采用实时定量PCR (Quantitative real-time PCR, QRT-PCR)和Western blot法对kras基因mRNA和蛋白表达分别进行检测。结果表明，胸腺淋巴瘤kras基因mRNA表达水平（2.66 ± 1.51）比正常胸腺组织（0.93 ± 0.30）明显增高，两组比较有统计学意义（,p,<,0.05），胸腺淋巴瘤kras基因蛋白表达水平也高于正常胸腺组织。结果提示，kras基因与辐射诱发小鼠胸腺淋巴瘤密切相关，可能是辐射致癌的一种易感基因。

Abstract

In order to investigate the relation between kras gene and thymic lymphomas of mice induced by X-rays, we made the thymic lymphomas model of BALB/c mice exposed to X-rays, so as to extracte total RNA and synthesize cDNA and total protein from both of thymic lymphomas and normal thymus tissue. The mRNA and protein expression of kras gene in thymic lymphomas and normal thymus were detected by QRT-PCR and Western blot, respectively. The results show that mRNA expression of kras gene in thymic lymphomas (2.66 ± 1.51) is significantly higher (,p,<,0.05) than that of normal thymus tissue (0.93±0.30); and protein expression of kras gene in thymic lymphomas is higher than that of normal thymus tissue. The results suggest that kras gene might be relative to thymic lymphomas induced by X-rays, and it might be a susceptibility gene to radiation carcinogenesis.

关键词

胸腺淋巴瘤kras基因电离辐射实时定量PCR

Keywords

Thymic lymphomasKras genesIonizing radiationQuantitative real-time PCR

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

水凝胶基光催化复合材料的辐射制备研究进展

1~20 eV低能电子对DNA的多重损伤及其瞬态阴离子机制

水泥固化处理中水的存在形式对氢气辐照产额影响的研究进展

电离辐射促进神经胶质瘤细胞初级纤毛发生

电离辐射后肿瘤相关成纤维细胞中差异表达基因的生物信息学分析