用自旋标记技术观察离体与整体照射对大鼠红细胞膜巯基状态的影响

康鑫; 方允中; 忻文娟; 孙存普

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用自旋标记技术观察离体与整体照射对大鼠红细胞膜巯基状态的影响

更新时间：2023-04-23

- 用自旋标记技术观察离体与整体照射对大鼠红细胞膜巯基状态的影响
- OBSERVATIONS ON THE EFFECTS OF IRRADIATION IN VITRO AND IN VIVO ON THE SH-GROUP OF RAT ERYTHROCYTE MEMBRANE BY SPIN LABEL TECHNIC
- 辐射研究与辐射工艺学报 1990年8卷第2期页码：103-106
- 作者机构：
  
  1.军事医学科学院放射医学研究所，北京
  *.中国科学院生物物理研究所
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康鑫, 方允中, 忻文娟, 等. 用自旋标记技术观察离体与整体照射对大鼠红细胞膜巯基状态的影响[J]. 辐射研究与辐射工艺学报, 1990,8(2):103-106.

Kang Xin, Fang Yunzhong, Xin Wenjiuan, et al. OBSERVATIONS ON THE EFFECTS OF IRRADIATION IN VITRO AND IN VIVO ON THE SH-GROUP OF RAT ERYTHROCYTE MEMBRANE BY SPIN LABEL TECHNIC[J]. Journal of Radiation Research and Radiation Processing, 1990,8(2):103-106.
康鑫, 方允中, 忻文娟, 等. 用自旋标记技术观察离体与整体照射对大鼠红细胞膜巯基状态的影响[J]. 辐射研究与辐射工艺学报, 1990,8(2):103-106. DOI：

Kang Xin, Fang Yunzhong, Xin Wenjiuan, et al. OBSERVATIONS ON THE EFFECTS OF IRRADIATION IN VITRO AND IN VIVO ON THE SH-GROUP OF RAT ERYTHROCYTE MEMBRANE BY SPIN LABEL TECHNIC[J]. Journal of Radiation Research and Radiation Processing, 1990,8(2):103-106. DOI：

摘要

用MAL-6（2, 2, 6, 6-tetramethyl-4-maleimide-piperidin-1-oxyl）作为自旋标记物研究了电离辐射对大鼠红细胞膜蛋白的影响。本文探索到：（1）100—400 Gy离体照射对红细胞膜中自旋标记巯基显示的ESR波谱无明显影响。（2)8.5 Gy全身照射可使红细胞膜的ESR波谱中弱固定化与强固定化波峰高度之比（W/S）增加。上述结果表明了动物体或红细胞中内源性活性氧产生与清除的不平衡导致活性氧的损伤作用。

Abstract

MAL-6(2, 2,6,6,-tetra-methyl-4-malaimide-piperidin-1-oxyl) was used as spin label in studying the effects of ionizing radiation on rat erythrocyte membrane protein. By this approach, It was found out: (1). 100-400 Gy irradiation in vitro gave no obvious effects on ESR spectra revealed by spin label-SH group in the erythrocyte membrane. (2). Whole-body irradiation with 8.5 Gy caused increase of w/s ratio for the erythrocyte membrane.,It was suggested that the above-mentioned results might be attributed to the damage action of the endogeneous active oxygen species produced from imbalance between their generation and scavenging in erythrocyte or living body.

关键词

自旋标记膜蛋白巯基离体照射整体照射

Keywords

Spin labelSH group of membrane proteinIrradiation in vitro and in vivo

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