Shen Liying, Yuan Xingzheng, Gan Xiaoxian, et al. EFFECT OF 60Co γ-IRRADIATION ON THE INFECTIVITY OF TOXOPLASMA GONDII BRADYZOITES[J]. Journal of Radiation Research and Radiation Processing, 1992,10(4):246-248.
Shen Liying, Yuan Xingzheng, Gan Xiaoxian, et al. EFFECT OF 60Co γ-IRRADIATION ON THE INFECTIVITY OF TOXOPLASMA GONDII BRADYZOITES[J]. Journal of Radiation Research and Radiation Processing, 1992,10(4):246-248.DOI:
Homogenates of mouse brains harbouring toxoplasma NT strain cysts were irradiated at various doses of ,60,Co ,γ, ray and digested with 0.4% trypsin buffer to release bradyzoites from cysts. The suspensions containing various number bradyzoites were obtained through a serial 10-fold dilution in saline solution and inoculated intraperitoneally into mice separately. Infectivity of toxoplasma bradyzoites was determined by bioassay of mice. A group of mice inoculated with non-irradiated bradyzoites was used as control. Results showed that the minimal effective dose of γ rays controlling infectivity of toxoplasma NT strain bradyzoites was 0.55 kGy. Effect of 0.1 kGy ,γ, rays on infectivity of toxoplasma bradyzoites was not significant. Infectivity of bradyzoites irradiated at 0.45 kGy had 10,4, fold reduction in comparison with that of 0.1 kGy.