A CHEMILUMINESCENT METHOD FOR DETERMINATION OF LIPID PEROXIDATION

LIANG Xiaofeng; HU Tianxi; FAN Xiaobing

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A CHEMILUMINESCENT METHOD FOR DETERMINATION OF LIPID PEROXIDATION

更新时间：2023-03-30

- A CHEMILUMINESCENT METHOD FOR DETERMINATION OF LIPID PEROXIDATION
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 1, Pages: 40-44(2003)
- 作者机构：
  
  1.上海交大昂立公司生物医药研究所　上海　200030
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LIANG Xiaofeng, HU Tianxi, FAN Xiaobing. A CHEMILUMINESCENT METHOD FOR DETERMINATION OF LIPID PEROXIDATION. [J]. Journal of Radiation Research and Radiation Processing 21(1):40-44(2003)
DOI：

LIANG Xiaofeng, HU Tianxi, FAN Xiaobing. A CHEMILUMINESCENT METHOD FOR DETERMINATION OF LIPID PEROXIDATION. [J]. Journal of Radiation Research and Radiation Processing 21(1):40-44(2003) DOI：

摘要

建立了一个测定脂质过氧化（Lipid peroxidation，LPO）的化学发光体系，测试某些抗氧化剂清除脂自由基的活性。取含有不饱和脂肪酸的脂质加入到测量管中，再加入鲁米诺及双重蒸馏水，放入预恒温箱中37℃预温育0.5h，取出并加入启动剂AAPH {2,2’-azobis (2-amidinopropane) dihydrochloride}，立即放入预先温度设至38—39℃的生化发光测量仪中，用T-2程序测定每6s钟的脉冲数（CP6s），连续测定，观察化学发光（chemiluminescence, CL）的动力学变化和是否产生脂质过氧化，随后加入各类抗氧化剂，根据实际需要，选其某一次的CL强度作为评判指标。建立了一个应用鲁米诺化学发光法测定脂质过氧化的体系并对比了多种抗氧化剂的活性。结果发现，Vit.C、茶多酚（EGCG）、还原型谷胱甘肽（GSH）皆有抑制CL，即抗脂质过氧化的作用。该方法是一种快速、灵敏、简便的测定脂质过氧化及筛选抗氧化剂的新方法。

Abstract

We established a chemiluminescent system for determination of lipid peroxidation and screening antioxidants. The lipid containing unsaturated fatly acids was injected into a glass tube. Luminol solution and the deionized water were added into it too. The glass tube was put into a preincubation box to incubate it for 0.5h at 37℃. AAPH solution was injected into the tube for immediate measurement in a biochimiluminometer at 38—39℃. The pulses / 6s (CP6s) were determined with T-2 program. Chemiluminescent dynamic and lipid peroxidation changes were observed continuously. Once the CL intensity of lipid peroxidation got peak, the antioxidant which has different concentration was added immediately in situ. A certain CL intensity (CP6s) was chosen as evaluation index to compare the activity of antioxidants. A luminol chemiluminescent system for determination of lipid peroxidation has been made. It was found that Vit.C, teapolyphenol, and glutathione have effects on scavenging lipid free radicals. The new method is quick, sensitive, and simple for determination of lipid peroxidation and screening antioxidants.

关键词

化学发光脂质过氧化抗氧化剂

Keywords

ChemiluminescenceLipid peroxidationAntioxidants

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