EFFECT OF <sup>60</sup>CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN <italic style="font-style: italic">Deinococcus radiodurans</italic>

SUN Cuifeng; LIU Fenju; KONG Xiangrong; CHEN Jian; NING Ping

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EFFECT OF ⁶⁰CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans

更新时间：2023-03-30

- EFFECT OF ⁶⁰CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 2, Pages: 157-160(2003)
- 作者机构：
  
  1.苏州大学放射生物学教研室　苏州　215007
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SUN Cuifeng, LIU Fenju, KONG Xiangrong, et al. EFFECT OF ⁶⁰CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans. [J]. Journal of Radiation Research and Radiation Processing 21(2):157-160(2003)
DOI：

SUN Cuifeng, LIU Fenju, KONG Xiangrong, et al. EFFECT OF ⁶⁰CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans. [J]. Journal of Radiation Research and Radiation Processing 21(2):157-160(2003) DOI：

摘要

研究了耐辐射菌对,60,Coγ射线的辐射抗性，观察不同剂量照射后，细菌中蛋白质含量、辐照剂量及照射后培养不同时间的关系。应用平板菌落计数法，计数不同剂量辐照后的克隆数，计算存活率，绘制剂量—存活曲线。采用紫外分光光度法检测细菌中蛋白质的含量。结果表明, 耐辐射菌的存活曲线呈肩型，具有极强的辐射抗性。蛋白质的含量随着照射剂量的升高而不断增加，当辐射剂量达到5kGy时，蛋白质含量最高(p,<,0.01)；若受照剂量,>,5kGy时，则蛋白质含量随受照射剂量的升高而逐渐降低。5kGy辐照后，随照射后温育时间的延长，蛋白质含量不断降低，培养时间为6h，蛋白质的含量最低(p,<,0.01),与对照组(未受照射组)相比，无显著性差异(p,>,0.05)。

Abstract

In this paper, the radioresistance of bacterium ,Deinococcus radiodurans, to ,60,Co γ-rays and the relationship between the protein content in the bacterium and the irradiation dose as well as the time after irradiation were studied. By counting the colonies on the plates which grew up from the irradiated and the control bacterium, the survival rate at each irradiation dose was calculated. The protein concentration was measured with the ultraviolet spectrophotometry. The results showed that the typical survival curve of ,Deinococcus radiodurans, had a wide shoulder up to 5kGy, characterizing the radioresistance of the bacterium. The protein concentration increased with increasing dose, reaching the maximum at 5kGy. With time prolongation after 5kGy irradiation, the protein expressed in the bacterium reduced. And the lowest protein concentration, basically the same as the control, was observed 6h after the irradiation.

关键词

耐辐射球菌辐射抗性60Co γ射线蛋白质含量

Keywords

Deinococcus radioduransRadioresistance60Co γ-rayProtein concentration

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Institute of Radiation Medicine, Suzhou Medical College

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EFFECT OF 60CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans

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EFFECT OF ⁶⁰CO γ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans