Effect of LexA protein in <italic style="font-style: italic">Deinococcus radiodurans</italic> on DNA strand breaks by <sup>60</sup>Coγ-rays

LIU Fenju; SUN Cuifeng; CHEN Jian; NING Ping

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Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by ⁶⁰Coγ-rays

更新时间：2023-03-30

- Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by ⁶⁰Coγ-rays
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 2, Pages: 111-113(2004)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215007
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LIU Fenju, SUN Cuifeng, CHEN Jian, et al. Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by ⁶⁰Coγ-rays. [J]. Journal of Radiation Research and Radiation Processing 22(2):111-113(2004)
DOI：

LIU Fenju, SUN Cuifeng, CHEN Jian, et al. Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by ⁶⁰Coγ-rays. [J]. Journal of Radiation Research and Radiation Processing 22(2):111-113(2004) DOI：

摘要

观察不同剂量γ射线、不同细胞数量和不同浓度的LexA蛋白对DNA链断裂与修复的影响。结果表明，在2—10Gy范围内，照射剂量与DNA断裂程度之间存在良好的线性关系，,r,=0.881；当细胞浓度为（OD,600, =0.4—0.6，相当于2×10,8,细胞/ml），细胞液体积为0.1—0.5ml时，细胞DNA含量与相对荧光强度成正相关；结论抗辐射菌LexA蛋白对γ射线所致细胞DNA链断裂无明显的保护作用。

Abstract

The results indicate that in 2—10Gy range of the γ-ray irradiation, a linear relations (,r,=0.881) were found between the irradiation dose and DNA concentration,. When the number of cells was 8×10,7,—10×10,7, the concentration of DNA cells related linearly, too, with relative fluorometric intensity. Difference in concentrations of the LexA protein showed no significant protection on DNA strand breaks caused by the γ-rays.

关键词

FADULexA蛋白DNA链断裂60Co γ射线

Keywords

FADULexADNA strand breaks60Coγ-rays

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Institute of Biophyscs, Academia Sinica

Department of Environmental Sciences, Shanxi University

Shanghai 2nd Military Medical College

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Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by 60Coγ-rays

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Effect of LexA protein in Deinococcus radiodurans on DNA strand breaks by ⁶⁰Coγ-rays