AFM observation of DNA molecules and their double strand fragments brokenby α-particles

NI Meinan; ZHAO Kui; SUI Li; MEI Junping; GUO Jiyu; WANG Ruyu

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AFM observation of DNA molecules and their double strand fragments brokenby α-particles

更新时间：2023-03-30

- AFM observation of DNA molecules and their double strand fragments brokenby α-particles
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 2, Pages: 106-110(2004)
- 作者机构：
  
  1.中国原子能科学研究院核物理研究所　北京　102413
  2.北京师范大学射线束技术与材料改性教育部重点实验室　北京　100875
  3.河北工业大学　天津　300130
  4.中国科学院理化技术研究所　北京　100101
- 作者简介：
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NI Meinan, ZHAO Kui, SUI Li, et al. AFM observation of DNA molecules and their double strand fragments brokenby α-particles. [J]. Journal of Radiation Research and Radiation Processing 22(2):106-110(2004)
DOI：

NI Meinan, ZHAO Kui, SUI Li, et al. AFM observation of DNA molecules and their double strand fragments brokenby α-particles. [J]. Journal of Radiation Research and Radiation Processing 22(2):106-110(2004) DOI：

摘要

DNA是生命信息的载体，是辐射生物学效应最重要的靶分子。研究DNA辐射损伤机理，需掌握观测DNA分子及其双链断裂（Double Strand Breaks，DSB）碎片的技术。在大气状态下，使用原子力显微镜（AFM）技术获得了3种经提纯的DNA分子的直观图像。在水溶液中，利用,241,Am-α源辐照装置对其中一种DNA分子作低剂量照射，首次获得了DNA双链断裂碎片的AFM图像。为研究不同类型辐射—特别是重离子辐射—导致的DNA双链断裂几率的统计模型，做了技术准备。

Abstract

DNA , the carrier of bio-information, is the most important target molecule in radiobiology. In order to understand the DSB mechanism of DNA molecules induced by ionizing radiations, the structure and property of DNA molecule and its DSB fragment shall be studied. In this paper, three kinds of purified DNA molecules were observed by Tapping Mode AFM in atmosphere. One kind of the DNA molecules in water solution was irradiated by ,241,Am-α source irradiation device. Intuitive images of the molecules and DSB fragments were obtained. By this way, further study of statistic model of probability of the DSB of DNA molecules induced by various radiations, especially heavy ions, could be carried out.

关键词

DNAAFM生物样品吸附AFM针尖展宽效应双链断裂

Keywords

DNAAFMBio-sample AdsorptionAFM Tip Broadening EffectDouble Strand Breaks

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