Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line

SHEN Bo; SHEN Bing

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Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line

更新时间：2023-03-30

- Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 1, Pages: 45-48(2005)
- 作者机构：
  
  1.吉林大学公共卫生学院卫生部放射生物重点实验室　长春　130021
  2.中国辐射防护研究院科技信息中心　太原　030006
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SHEN Bo, SHEN Bing. Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line. [J]. Journal of Radiation Research and Radiation Processing 23(1):45-48(2005)
DOI：

SHEN Bo, SHEN Bing. Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line. [J]. Journal of Radiation Research and Radiation Processing 23(1):45-48(2005) DOI：

摘要

对γ射线诱导中国仓鼠成纤维（CHL）细胞基因组不稳定性进行了研究。利用CB法微核检测技术对一次照射、二次照射的CHL细胞进行微核测定，观察DNA损伤程度的变化情况。结果显示，微核发生率与细胞受照剂量呈相关关系，并随培养时间延长呈下降趋势。细胞再次受照后，其微核发生率与一次受照剂量呈相关关系。表明微核发生率与染色体损伤程度存在剂量相关性，微核检测技术可以用于生物效应表现为染色体损伤的辐射诱导基因组不稳定性的研究。

Abstract

To analyze the genomic instability induced by γ-rays, cytokinesis-block MN assay was employed to investigate the gene damage of cells and their progeny cells exposed to radiation. The micronucleus frequency were related to the irradiation dose and decreased as the culture time lengthened. There was a correlation between the micronucleus frequency and the initial dose after the second irradiation. The micronucleus frequency may be causally related to radiation-induced genomic instability which is expressed as chromosomal damage leading to micronuclei.

关键词

基因组不稳定性CB法微核检测技术

Keywords

Genomic instabilityCytokinesis-block MN assay

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Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity

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