Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation

XIA Jingguang; LI Wenjian; WANG Jufang; GUO Chuanling; JIN Xiaodong; GAO Qingxiang

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Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation

更新时间：2023-03-30

- Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 1, Pages: 49-52(2005)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.中国科学院研究生院　北京　100049
  3.兰州大学生命科学学院　兰州　730000
- 作者简介：
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XIA Jingguang, LI Wenjian, WANG Jufang, et al. Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation. [J]. Journal of Radiation Research and Radiation Processing 23(1):49-52(2005)
DOI：

XIA Jingguang, LI Wenjian, WANG Jufang, et al. Effects of low priming dose irradiation on cell cycle arrest of HepG2 cells caused by high dose irradiation. [J]. Journal of Radiation Research and Radiation Processing 23(1):49-52(2005) DOI：

摘要

以人肝癌细胞hepG2为研究对象，用流式细胞术测定并分析了低剂量γ射线（5cGy）预照射对高剂量照射（3Gy）诱导的细胞周期阻滞的影响。结果显示：（1）低剂量照射可引起hepG2细胞在G,2,/M期短暂累积（至照射后4h）；（2）低剂量照射促进hepG2细胞的生长；（3）高剂量照射后，hepG2细胞的G,2,期发生阻滞，而S期只发生短暂延迟；（4）与单纯高剂量照射相比，低剂量照射预处理后4h给予高剂量照射可进一步促进hepG2细胞在G,2,/M期累积，但预处理后8h给予高剂量可促进细胞通过G,2,/M期。实验结果表明，低剂量照射预处理对高剂量照射诱导的细胞周期阻滞的影响，取决于两次照射的时间间隔。

Abstract

Human hepatoma cells hepG2 were irradiated twice by ,60,Co γ-rays with a priming dose of 5cGy and a higher dose of 3Gy performed 4h or 8h after the low dose irradiation. Effects of the priming dose irradiation on cell cycle arrest caused by high dose were examined with flow cytometry. Cells in G,2,/M phase accumulated temporarily after the 5cGyirradiation, and proliferation of tumor cells was promoted significantly by the low dose irradiation. After the 3Gy irradiation, G,2, phase arrest occurred, and S phase delayed temporally. In comparison with 3kGy irradiation only, the priming dose delivered 4h prior to the high dose irradiation facilitated accumulation of hepG2 cells in G,2,/M phase, whereas the priming dose delivered 8h prior to the high dose irradiation helped the cells to overcome G,2, arrest. It was concluded that effects of the priming dose treatment on cell cycle arrest caused by high dose irradiation were dependent on time interval between the two irradiations.

关键词

低剂量辐射细胞周期阻滞肝癌细胞

Keywords

Low dose radiationCell cycle arrestHepatoma cells

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