Analysis of proteomic changes of the serum of irradiated mice

WANG Zhidong; CHEN Xiaohua; DONG Bo; ZHANG Junquan; RAO Yalan; GAO Ronglian; HOU Lili; MAO Bingzhi

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Analysis of proteomic changes of the serum of irradiated mice

更新时间：2023-03-30

- Analysis of proteomic changes of the serum of irradiated mice
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 1, Pages: 53-56(2005)
- 作者机构：
  
  1.军事医学科学院放射医学研究所　北京　100850
- 作者简介：
- 基金信息：
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WANG Zhidong, CHEN Xiaohua, DONG Bo, et al. Analysis of proteomic changes of the serum of irradiated mice. [J]. Journal of Radiation Research and Radiation Processing 23(1):53-56(2005)
DOI：

WANG Zhidong, CHEN Xiaohua, DONG Bo, et al. Analysis of proteomic changes of the serum of irradiated mice. [J]. Journal of Radiation Research and Radiation Processing 23(1):53-56(2005) DOI：

摘要

为寻找急性放射病早期诊断指标和新的治疗靶点，探讨辐射损伤的发病机理，采用蛋白质组学的双向电泳和蛋白质氨基酸序列分析技术研究了8Gy γ射线照射后24h小鼠血清蛋白质的变化，鉴定有差异的蛋白质，并用蛋白质印迹（Western blotting）方法进行验证。结果显示，双向电泳发现8Gy照射后24h小鼠血清有一显著变化蛋白点，分子量约为15ku，经氨基酸序列分析发现为结合珠蛋白（Haptoglobin，Hp）的α亚单位。Western blotting方法进一步验证了双向电泳的结果。结果表明，经8Gy γ射线照射后24h小鼠血清中结合珠蛋白发生显著增加，可能在辐射损伤中发挥着一定的作用，此结果为探索急性放射损伤的发病机理提供了新的线索。

Abstract

To explore the early diagnostic factors, new therapeutic targets and mechanisms of acute radiation disease. Proteomic changes of the serum of irradiated mice were studied using 2-DE and Q-TOF-MS approaches. One higher level expressed protein after the irradiation was found, and it was identified as α chain of haptoglobin by Q-TOF–MS. We confirmed the result by Western blotting with anti-haptoglobin antibody. Haptoglobin may involve in the process of acute radiation injury.

关键词

急性放射损伤双向电泳正交加速电喷雾串联质谱结合珠蛋白

Keywords

Acute radiation injury2DEQ-TOF-MSHhaptoglobin

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