P21 protein expressing induced by ionizing radiation and its iffect in cell cycle uncoupling

JU Guizhi; YAN Fengqin; FU Shibo; LI Pengwu

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P21 protein expressing induced by ionizing radiation and its iffect in cell cycle uncoupling

更新时间：2023-03-30

- P21 protein expressing induced by ionizing radiation and its iffect in cell cycle uncoupling
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 4, Pages: 251-253(2005)
- 作者机构：
  
  1.吉林大学公共卫生学院卫生部放射生物学重点实验室　长春　130021
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JU Guizhi, YAN Fengqin, FU Shibo, et al. P21 protein expressing induced by ionizing radiation and its iffect in cell cycle uncoupling. [J]. Journal of Radiation Research and Radiation Processing 23(4):251-253(2005)
DOI：

JU Guizhi, YAN Fengqin, FU Shibo, et al. P21 protein expressing induced by ionizing radiation and its iffect in cell cycle uncoupling. [J]. Journal of Radiation Research and Radiation Processing 23(4):251-253(2005) DOI：

摘要

采用免疫细胞化学方法检测P21蛋白表达的变化。采用PI荧光标记及流式细胞术（Flow cytometry，FCM）测定细胞周期并分析细胞倍体的变化。观察电离辐射对EL−4细胞P21蛋白表达的影响及对细胞周期解偶联的作用。时效实验结果显示，4.0Gy X射线照射后2—72 h，EL−4细胞中P21蛋白表达明显增高（,p,<,0.05—,p,<,0.001）。量效实验结果显示，0.5—6.0Gy X射线照射后24 h，EL−4细胞中P21蛋白表达明显增高（,p,<,0.01—,p,<,0.001）。结果还显示，1.0—6.0Gy X射线照射后，EL−4细胞的8倍体细胞数在各剂量组均未见明显变化（均,p,>,0.05）。研究表明，电离辐射可诱导EL−4细胞P21蛋白表达，但不诱导EL−4细胞周期解偶联。提示P21蛋白可能参与细胞周期解偶联的分子调控。

Abstract

The work is to investigate whether P21 protein expression could be induced by ionizing radiation and the effect on cell cycle uncoupling. Immunocytochemistry assay was used to measure P21 protein expression. Polyploid was analyzed by FCM following staining of cells with propidium iodide (PI). The results showed that P21 protein expression of EL−4 cells was increased significantly 2—72h after 4.0Gy X−ray irradiation (,p,<,0.05—,p,<,0.001). In dose−effect experiments it was found that P21 protein expression also increased significantly 24 h after exposure to 0.5—6.0Gy X−rays of EL−4 cells (,p,<,0.01—,p,<,0.001). However, 8N cells were not changed in EL−4 cells irradiated to 1.0—6.0Gy by X−rays compared with sham−irradiated control (,p>,0.05). These results demonstrated that the ionizing radiation could induce P21 protein expression, but not cell cycle uncoupling. It suggested that P21 might play an important role in cell cycle uncoupling.

关键词

电离辐射P21蛋白细胞周期解偶联

Keywords

Ionizing radiationP21 protein expressionCell cycle uncoupling

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