Effects of mitomycin(MMC) and γ−ray irradiation on cytochome p450 content and activities of CYP2B1and CYP2E1 in rats

YAN Minfen; HAO Furong; XU Liming; TONG Shungao; JI Huajun; SHEN Zhifen; JIN Yizun

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Effects of mitomycin(MMC) and γ−ray irradiation on cytochome p450 content and activities of CYP2B1and CYP2E1 in rats

更新时间：2023-03-30

- Effects of mitomycin(MMC) and γ−ray irradiation on cytochome p450 content and activities of CYP2B1and CYP2E1 in rats
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 4, Pages: 246-250(2005)
- 作者机构：
  
  1.复旦大学放射医学研究所　上海　200032
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YAN Minfen, HAO Furong, XU Liming, et al. Effects of mitomycin(MMC) and γ−ray irradiation on cytochome p450 content and activities of CYP2B1and CYP2E1 in rats. [J]. Journal of Radiation Research and Radiation Processing 23(4):246-250(2005)
DOI：

YAN Minfen, HAO Furong, XU Liming, et al. Effects of mitomycin(MMC) and γ−ray irradiation on cytochome p450 content and activities of CYP2B1and CYP2E1 in rats. [J]. Journal of Radiation Research and Radiation Processing 23(4):246-250(2005) DOI：

摘要

为研究3Gy γ射线全身照射和丝裂霉素C （Mitomycin C，MMC）对雄性（Sprague-Dawley，SD）大鼠肝脏细胞色素P450含量、CYP2B1、CYP2E1活性的影响，分别给大鼠腹腔注射1mg/kg d 的MMC（分1d，连续3d及6d用药三组），3mg/kg 的MMC 1d，3Gy γ射线全身照射，3Gy γ射线全身照射加1d 3mg/kg MMC，另设空白对照和溶剂对照。各组处理结束后24h，处死大鼠取肝脏，制备微粒体，测P450含量、CYP2B1、CYP2E1活性。实验结果表明，给MMC 1mg/kg d，1d后P450含量、CYP2B1活性变化无统计学差异（,p,>,0.05），CYP2E1活性明显上升（,p,<,0.01）；连续3 d或6 d后，P450含量、CYP2B1、CYP2E1活性均无明显变化（,p,>,0.05）。给3mg/kg 的MMC 1d，对P450含量、CYP2B1活性影响无统计学差异（,p,>,0.05），CYP2E1活性上升明显（,p,<,0.01）；3Gy γ射线全身照射后，P450含量、CYP2B1活性无明显变化（,p,>,0.05），CYP2E1活性下降（,p,<,0.05）；分析发现，3mg/kg MMC与3Gy γ射线之间没有交互作用。结果提示，γ射线可以抑制雄性SD大鼠肝脏CYP2E1活性，MMC对CYP2E1活性有诱导作用，但多次刺激使其耐受，P450含量、CYP2B1活性对γ射线、MMC不敏感。

Abstract

In this paper, content of cytochrome P450 and activities of CYP2B1and CYP2E1 in liver of male SD rats subjected to intraperitioned injection of MMC or γ−ray irradiation, were studied. Groups No.1-3 of the rats were injected with MMC of 1mg/kg d for 1, 3 or 6days. Group No.4-6 of the rats were treated respectively with one injection of MMC at 3mg/kg, 3Gy of ,137,Cs γ−ray irradiation, or a combination of the MMC injection at 3mg/kg after 3Gy γ−ray irradiation. Biological measurements of the experimental groups were compared to blank groups and solvent control groups. The results showed that for the group administered on the first day with MMC at 1mg/kg, the CYP2E1 activity increased (,p,<,0.01), whereas the P450 content and the CYP2B1 activity did not change. For the group injected with MMC at 1mg/kg d for 3d or 6d, no significant changes were found in the P450 content and the activities of CYP2B1and CYP2E1. With the 3mg/kg MMC group, the CYP2E1 activity increased significantly (,p,<,0.01), but no marked effects were observed in the P450 content and the CYP2B1 activity. The CYP2E1 activity deceased in the 3Gy irradiated group (,p,<,0.05), with no effect on the P450 content and the CYP2B1 activity. There was no interaction between 3mg/kg MMC and 3Gy irradiation. The results indicated that MMC induced the CYP2E1 activity, yet repeated treatment with MMC might cause the rats to gain some resistance to it, in addition, 3Gy γay−irradiation inhibited the CYP2E1activity, but combined treatments of the 3Gy irradiation and MMC injection showed no significant effect on the P450 content and the CYP2B1 activity in vivo.

关键词

γ照射丝裂霉素CP450CYP2B1CYP2E1

Keywords

γ−irradiationMMCP450CYP2B1CYP2E1

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Effect of γ-irradiation and mitomycin C (MMC) on CYP2B1 and CYP2E1 activities in rat hepatic microsomes in vitro

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