Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses

JI Xinxin; HOU Wugang; ZHAO Jie; ZHAO Yong; LI Wei; ZHANG Yuanqiang

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Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses

更新时间：2023-03-30

- Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 6, Pages: 362-366(2006)
- 作者机构：
  
  1.第四军医大学基础部组织学与胚胎学教研室　西安　710032
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JI Xinxin, HOU Wugang, ZHAO Jie, et al. Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses. [J]. Journal of Radiation Research and Radiation Processing 24(6):362-366(2006)
DOI：

JI Xinxin, HOU Wugang, ZHAO Jie, et al. Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses. [J]. Journal of Radiation Research and Radiation Processing 24(6):362-366(2006) DOI：

摘要

探讨转化生长因子-β（Transforming growth factor-β，TGF-β）超家族细胞内信号转导分子Smads在电磁脉冲辐射后成年小鼠睾丸中的表达变化及其意义。采用场强为400kV/m的辐射场对成年Balb/c小鼠进行全身照射，于照射后1、7、14、21、28d分别取小鼠睾丸组织制备标本，免疫组织化学ABC法检测Smad4、Smad1和Smad5的表达及变化，并通过图像分析系统以及统计学软件对实验结果进行统计学分析。结果显示辐射组小鼠Smad4、Smad1和Smad5在睾丸中的表达部位发生了细胞和时期特异性的变化，表达强度也发生了显著变化。结果提示Smad4、Smad1和Smad5及其介导的信号转导通路在小鼠睾丸辐射损伤及其修复过程中可能发挥了重要作用。

Abstract

Changes in expressions of smad4, smad1 and smad5 in the spermatogenic cells of mice after whole-body exposure to electromagnetic pulse (EMP) were studied to elucidate possible mechanisms of the EMP-induced injuries. Twenty adult Balb/c mice were irradiated with 400kV/m EMP to the whole-body. Specimens were harvested on 1, 7, 14, 21and 28 days after the irradiation. Changes in cellular localizations and expressions of Smad4, Smad1 and Smad5 were examined by immunohistochemical ABC method. Quantitative analysis of the immunostaining was made by an image analysis system. In the seminiferous tubules, the localization of Smad4, Smad1 and Smad5 in the EMP group were significantly different from the control group. The expression densities of smad4, smad1 and smad5 changed significantly on 1, 7, 14, 21and 28 days after irradiation. The study indicates that Smad4, Smad1, Smad5 and BMPs/Smad signal pathway may play an important role in the process of impairment and repair of mouse testis irradiated by EMP.

关键词

Smads骨形成蛋白电磁脉冲小鼠睾丸

Keywords

SmadBMPsElectromagnetic pulse (EMP)MouseTestis

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Department of Radiation Medicine, Fourth Military Medical University

Department of Dentistry and Clinical Medicine; Fourth Military Medical University

Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University

Department of Urology, Xijing Hospital, Fourth Military Medical University

Department of Human Anatomy and Histoembryology, Fourth Military Medical University

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